Testing Data
(Total lysates (15 µg protein) from E. coli BL21 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:100000 diluted (0.005 µg/mL) purified anti-E. coli RNA Polymerase beta antibody (clone 8RB13). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). Ponceau S staining (lower) was used as loading control. Lane M: Molecular weight ladder. )
Mouse RNA Polymerase beta Monoclonal Antibody
Purified anti-E. coli RNA Polymerase beta Antibody
Reactivity
E Coli, Bordetella, Chlamydiae, Myxococcus, Salmonella, Vibrio
Applications
Western Blot, ELISA, Immunoprecipitation
Synonyms
RNA Polymerase beta; Monoclonal Antibody; Purified anti-E. coli RNA Polymerase beta Antibody; RNAP subunit beta; rpoB; groN; nitB; rif; ron; stl; stv; tabD; RNA polymerase subunit beta; Transcriptase subunit beta; anti-RNA Polymerase beta antibody
Host
Mouse
Reactivity
E Coli, Bordetella, Chlamydiae, Myxococcus, Salmonella, Vibrio
Clonality
Monoclonal
Isotype
Mouse IgG2b, kappa
Clone Number
8RB13
Form/Format
0.22 um filtered protein solution is in phosphate-buffered solution, pH 7.2.
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for anti-RNA Polymerase beta antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
WB - Quality tested; ELISA, IP - Reported in the literature, not verified in house
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 ug per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription. Additional reported applications (for the relevant formats) include: immunoprecipitation . The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 ug per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription. Additional reported applications (for the relevant formats) include: immunoprecipitation . The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS
Immunogen
Purified recombinant E. coli RNA polymerase beta subunit.
Structure
1252 amino acids with a predicted molecular weight of approximately 150 kD.
Preparation
The antibody was purified by affinity chromatography.
Distribution
Bacteria
Preparation and Storage
The antibody solution should be stored undiluted between 2°C and 8°C. May be sub-aliquoted and stored at -20°C to avoid multiple freeze thaw cycle
Testing Data
(Total lysates (15 µg protein) from E. coli BL21 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:100000 diluted (0.005 µg/mL) purified anti-E. coli RNA Polymerase beta antibody (clone 8RB13). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). Ponceau S staining (lower) was used as loading control. Lane M: Molecular weight ladder. )
Testing Data
(Total lysates (15 µg protein) from E. coli BL21 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:100000 diluted (0.005 µg/mL) purified anti-E. coli RNA Polymerase beta antibody (clone 8RB13). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). Ponceau S staining (lower) was used as loading control. Lane M: Molecular weight ladder. )
Related Product Information for anti-RNA Polymerase beta antibody
RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The beta subunit is the second-largest subunit, and is encoded by the roB gene. RNA polymerase beta is a subunit which, along wtih alpha (rpoC), limits core polymerase expression.
Product Categories/Family for anti-RNA Polymerase beta antibody
References
1. Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155.
2. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
3. Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB)
1. Olivencia B,et al. 2017. Sci Rep.. 10.1038/s41598-017-14410-z. PubMed
2. Wipperman MF et al. 2018. Molecular cell. 72(1):152-161 . PubMed
2. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
3. Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB)
1. Olivencia B,et al. 2017. Sci Rep.. 10.1038/s41598-017-14410-z. PubMed
2. Wipperman MF et al. 2018. Molecular cell. 72(1):152-161 . PubMed
Similar Products
Product Notes
The RNA Polymerase beta (Catalog #AAA680139) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Purified anti-E. coli RNA Polymerase beta Antibody reacts with E Coli, Bordetella, Chlamydiae, Myxococcus, Salmonella, Vibrio and may cross-react with other species as described in the data sheet. AAA Biotech's RNA Polymerase beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB - Quality tested; ELISA, IP - Reported in the literature, not verified in house Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 ug per ml. It is recommended that the reagent be titrated for optimal performance for each application. Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription. Additional reported applications (for the relevant formats) include: immunoprecipitation. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS. Researchers should empirically determine the suitability of the RNA Polymerase beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNA Polymerase beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
