Western Blot (WB)
(Western Blot detection against Immunogen (37.99kD).)
Mouse anti-Human RING Finger Protein 25 Monoclonal Antibody | anti-RNF25 antibody
RING Finger Protein 25 (RNF25, E3 Ubiquitin-protein Ligase RNF25, AO7) (HRP)
Gene Names
RNF25; AO7
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RING Finger Protein 25; Monoclonal Antibody; RING Finger Protein 25 (RNF25; E3 Ubiquitin-protein Ligase RNF25; AO7) (HRP); EC=6.3.2.-; anti-RNF25 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1D10
Specificity
Recognizes human RNF25.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Applicable Applications for anti-RNF25 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa352-460 from human RNF25 (NP_071898) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
RRHPKGGECHAPKGTRDTQELPPPEGPLKEPMDLKPEPHSQGVEGPPQEKGPGSWQGPPPRRTRDCVRWERSKGRTPGSSYPRLPRGQGAYRPGTRRESLGLESKDGS
Conjugate
HRP
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (37.99kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (37.99kD).)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to RNF25 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to RNF25 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)
Testing Data
(Detection limit for recombinant GST tagged RNF25 is 0.03ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged RNF25 is 0.03ng/ml as a capture antibody.)
Product Categories/Family for anti-RNF25 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,219 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF25
NCBI Official Synonym Full Names
ring finger protein 25
NCBI Official Symbol
RNF25
NCBI Official Synonym Symbols
AO7
NCBI Protein Information
E3 ubiquitin-protein ligase RNF25; ring finger protein AO7
UniProt Protein Name
E3 ubiquitin-protein ligase RNF25
Protein Family
UniProt Gene Name
RNF25
UniProt Entry Name
RNF25_HUMAN
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Product Notes
The RNF25 rnf25 (Catalog #AAA6154692) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RING Finger Protein 25 (RNF25, E3 Ubiquitin-protein Ligase RNF25, AO7) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RING Finger Protein 25 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 3ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RNF25 rnf25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RING Finger Protein 25, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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