Mouse anti-Human Retinol-binding protein 4 Monoclonal Antibody | anti-RBP4 antibody

Mouse anti- human Retinol-binding protein 4(RBP4) monoclonal Antibody

Cat. Num. AAA1488158

• Gene Names: RBP4; RDCCAS; MCOPCB10
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G Purified
• Synonyms: Retinol-binding protein 4; Monoclonal Antibody; Mouse anti- human Retinol-binding protein 4(RBP4) monoclonal Antibody; Plasma retinol-binding protein; PRBP; RBP; RBP4; PRO2222; anti-RBP4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Specific for native and denatured forms
• Purity/Purification: >95%, Protein G Purified
• Sequence Length: 201

• Applicable Applications for anti-RBP4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant Human Retinol-binding protein 4 protein
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-48384 / sc-69795 / sc-46688
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: Mouse anti- human Retinol-binding protein 4 monoclonal antibody at 0.3ug/mlLane 1: Human positive serum1:10Lane 2: Human positive serum1:100Secondary:HRP labeled Goat polyclonal to Mouse IgG at 1/3000 dilutionPredicted band size: 23kdObserved band size: 27kd)

Western Blot (WB)

(All lanes: Mouse anti- human Retinol-binding protein 4 monoclonal antibody at 0.3ug/mlLane 1:RBP4 transfected E.coli lysateLane 2:Human positive serum1:100Lane 3:mouse liver tissueSecondary:HRP labeled Goat polyclonal to Mouse IgG at 1/3000 dilutionPredicted band size: 23kdObserved band size: 27kd)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human hepatic tissue using MBS1488158 at dilution of 1:200)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human nephridial tissue using MBS1488158 at dilution of 1:200)

Related Product Information for anti-RBP4 antibody

Retinol (Vitamin A) is transported in the blood bound to its carrier protein, retinol-binding protein (RBP), also designated plasma retinol-binding protein (PRBP) or RBP4. A member of the lipocalin family, RBP conveys retinol from stores in the liver to peripheral tissues. In plasma, RBP binds transthyretin (TTR, formerly called prealbumin) to prevent glomerular filtration of low molecular weight RBP in the kidneys. The stability of this complex holds importance because the molar ratio of RBP:TTR provides an indirect way to indicate marginal vitamin A deficiency. Vitamin A deficiency blocks the secretion of RBP resulting in defective delivery and supply to epidermal cells. Originally identified solely as a transporter protein, recent studies correlating increased levels of RBP expression in adipose tissue with insulin resistance have generated research into the possible roles the protein may play in the pathogenesis of type 2 diabetes and obesity.

NCBI and Uniprot Product Information

• NCBI GI #: 55743122
• NCBI GeneID: 5950
• NCBI Accession #: NP_006735.2
• NCBI GenBank Nucleotide #: NM_006744.3
• UniProt Accession #: P02753; O43478; O43479; Q5VY24; Q8WWA3; Q9P178; D3DR38
• Molecular Weight: 23,010 Da
• NCBI Official Full Name: retinol-binding protein 4
• NCBI Official Synonym Full Names: retinol binding protein 4
• NCBI Official Symbol: RBP4
• NCBI Official Synonym Symbols: RDCCAS; MCOPCB10
• NCBI Protein Information: retinol-binding protein 4
• UniProt Protein Name: Retinol-binding protein 4
• Protein Family: Retinol-binding protein
• UniProt Gene Name: RBP4
• UniProt Synonym Gene Names: PRBP; RBP
• UniProt Entry Name: RET4_HUMAN

NCBI Description

This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]

Uniprot Description

RBP4: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency). This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q23.33

Cellular Component: cytosol; extracellular region; extracellular space; protein complex

Molecular Function: protein binding; protein heterodimerization activity; retinal binding; retinol binding

Biological Process: cardiac muscle development; embryonic organ morphogenesis; embryonic retina morphogenesis in camera-type eye; embryonic skeletal development; eye development; fat-soluble vitamin metabolic process; female genitalia morphogenesis; gluconeogenesis; glucose homeostasis; heart development; lung development; maintenance of gastrointestinal epithelium; negative regulation of cardiac muscle cell proliferation; phototransduction, visible light; positive regulation of immunoglobulin secretion; positive regulation of insulin secretion; response to ethanol; response to retinoic acid; retinoid metabolic process; retinol metabolic process; urinary bladder development; uterus development; vagina development; visual perception; vitamin metabolic process

Disease: Microphthalmia, Isolated, With Coloboma 10; Retinal Dystrophy, Iris Coloboma, And Comedogenic Acne Syndrome