Mouse Retinol Binding Protein-1 (RBP1) Monoclonal Antibody | anti-RBP1 antibody

Retinol Binding Protein-1 (RBP1) Mouse Monoclonal Antibody

Cat. Num. AAA438071

• Gene Names: RBP4; RDCCAS; MCOPCB10
• Reactivity: Human, Chimpanzee, Monkey, Goat, Rabbit, Rat, Mouse.; Others not tested
• Applications: Immunofluorescence, Immunohistochemistry
• Synonyms: Retinol Binding Protein-1 (RBP1); Monoclonal Antibody; Retinol Binding Protein-1 (RBP1) Mouse Monoclonal Antibody; Cellular retinol-binding protein I; CRBP1; CRBP2; RBP1; RBP2; RBP4; RBPC; Retinol binding protein 1; Retinol binding protein 1 cellular; Retinol binding protein 2 cellular; Retinol binding protein 4 plasma; anti-RBP1 antibody

• Host: Mouse
• Reactivity: Human, Chimpanzee, Monkey, Goat, Rabbit, Rat, Mouse.; Others not tested
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: G4E4
• Specificity: This MAb recognizes an epitope within the 74-182 C-terminal sequence (11kD peptide fragment) of human serum Cellular Retinol Binding Protein 1 (CRBP 1), a single-chain glycoprotein belonging to the superfamily of hydrophobic molecule transporter proteins, which is responsible for transport of retinol (vitamin A1) from the liver to peripheral target tissues, like the eye, where it mediates the cellular uptake. CRBP 1 is synthesized by hepatic parenchymal cells where it becomes bound to its ligand retinol and is then released into the circulation, where it binds further to the protein transthyretin, to form a transporting complex, which is big enough not to be lost by filtration through the kidney glomeruli. It is detected in nearly all tissues with higher expression in adult ovary, pancreas, pituitary gland, adrenal gland, and fetal liver.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 201

• Applicable Applications for anti-RBP1 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasmic
• Immunogen: Retinol binding protein-1 purified from human plasma
• Hu-Chromosome Location: 10q23.33
• Positive Control: Hepatic or ovarian carcinoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with RBP1 (G4E4))

References

Reddy BM; Karande AA; Adiga PR. Antigenic determinants of human serum retinol binding protein as probed with monoclonal antibodies. Molecular Immunology, 1993, 30(15):1355-60. Reddy B. et al.: Biochem. Int. 21, 367-376 (1990). Reddy B. et al.: Molec. Immunol. 29, 511-516 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 1021087280
• NCBI GeneID: 5950
• NCBI Accession #: NP_001310446.1
• NCBI GenBank Nucleotide #: NM_001323517.1
• UniProt Accession #: P02753; O43478; O43479; Q5VY24; Q8WWA3; Q9P178; D3DR38
• Molecular Weight: 21-25kDa
• NCBI Official Full Name: retinol-binding protein 4 isoform a
• NCBI Official Synonym Full Names: retinol binding protein 4
• NCBI Official Symbol: RBP4
• NCBI Official Synonym Symbols: RDCCAS; MCOPCB10
• NCBI Protein Information: retinol-binding protein 4
• UniProt Protein Name: Retinol-binding protein 4
• Protein Family: RNA-binding protein
• UniProt Gene Name: RBP4
• UniProt Synonym Gene Names: PRBP; RBP
• UniProt Entry Name: RET4_HUMAN

NCBI Description

This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]

Uniprot Description

RBP4: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency). This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q23.33

Cellular Component: cytosol; extracellular region; extracellular space

Molecular Function: protein binding; retinol binding

Biological Process: cardiac muscle development; embryonic organ morphogenesis; embryonic retina morphogenesis in camera-type eye; embryonic skeletal development; eye development; female genitalia morphogenesis; gluconeogenesis; glucose homeostasis; heart development; lung development; maintenance of gastrointestinal epithelium; negative regulation of cardiac muscle cell proliferation; positive regulation of immunoglobulin secretion; positive regulation of insulin secretion; response to retinoic acid; retinoid metabolic process; retinol metabolic process; urinary bladder development; uterus development; vagina development

Disease: Microphthalmia, Isolated, With Coloboma 10; Retinal Dystrophy, Iris Coloboma, And Comedogenic Acne Syndrome