Mouse anti-Human Relaxin 3 Monoclonal Antibody | anti-RLN3 antibody

Relaxin 3 (H3 Relaxin, Insulin-Like Peptide-7, INSL7)

Cat. Num. AAA606600

• Gene Names: RLN3; H3; RXN3; ZINS4; insl7
• Reactivity: Human
• Applications: ELISA, Western Blot, Neutralization Assay
• Purity: Purified; Purified by Protein G affinity chromatography
• Synonyms: Relaxin 3; Monoclonal Antibody; Relaxin 3 (H3 Relaxin; Insulin-Like Peptide-7; INSL7); Anti -Relaxin 3 (H3 Relaxin; anti-RLN3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 8H147
• Specificity: Recognizes human Relaxin-3
• Purity/Purification: Purified; Purified by Protein G affinity chromatography
• Form/Format: Supplied as a powder lyophilized from sterile-filtered solution in PBS containing 5% trehalose. Reconstitute to 0.5mg/ml with 1ml sterile PBS.

• Applicable Applications for anti-RLN3 antibody: ELISA (EL/EIA), Western Blot (WB), Neutralization
• Application Notes: Suitable for use in Direct ELISA, Western Blot and Neutralization.; Dilution: Western Blot: 1ug/ml detected Relaxin 3 in recombinant human Relaxin 3 under non-reducing conditions only.; Neutralization: The ND50 is ~1-5ug/ml in the presence of 25ng/ml recombinant human Relaxin 3. Measured by its ability to neutralize Relaxin 3-induced cAMP production in the THP-1 human acute monocytic leukemia cell line (9).
• Immunogen: Recombinant corresponding to aa26-142 from human Relaxin 3 expressed in E. coli (Q8WXF3).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute with sterile ddH2O. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-RLN3 antibody

Human Relaxin 3 is one of seven relaxin-like peptides belonging to the insulin superfamily (1-4). Unlike human relaxins 1 and 2, it does not play a role in reproduction but appears to be a neuropeptide involved in stress response in the brain stem (3-5). The single-chain human Prorelaxin 3 shares 83% and 80% amino acid (aa) sequence identity with mouse and rat prorelaxin 3, respectively.The 142 aa Relaxin 3 pre-proprotein is processed to remove a 25aa signal peptide and a connecting peptide (aa53-118). The resulting mature Relaxin 3 is a 5.5kD, 51aa secreted heterodimer of A (aa119-142) and B (aa26-52) peptides connected by two intermolecular disulfide bonds (1). Mature human Relaxin-3 is 96%, 94%, and 92% aa identical to porcine, canine, and mouse Relaxin 3, respectively. This is much higher identity between species than that seen for other relaxins. Relaxin 3 is thus suggested to be the ancestral relaxin family member (2). Relaxin 3 is the only known ligand for the G-protein-coupled receptor GPCR135, designated RXFP3 (4, 6). In rodents, GPCR135 is expressed primarily in the supraoptic and paraventricular nucleus (6). This region has connections to the dorsal tegmental region of the pons (also called the nucleus incertus), where expression of Relaxin 3 is highest (5). Relaxin 3 also binds the more widely-expressed LGR7 (RXFP1) receptor, but with lower affinity than that of Relaxin 2 (1, 7). Although binding of Relaxin 3 to LGR7 increases intracellular cAMP, binding to GPCR135 inhibits cAMP accumulation, indicating coupling to Gi, Go or Gz by this receptor (1, 5). Relaxin 3 expression does not overlap well with its other receptor, GPCR142, which instead appears to be the primary receptor for INSL5 (3, 8).

Product Categories/Family for anti-RLN3 antibody

Antibodies; Abs to Hormones; Steroids

NCBI and Uniprot Product Information

• NCBI GI #: 18254464
• NCBI GeneID: 117579
• NCBI Accession #: NP_543140.1
• NCBI GenBank Nucleotide #: NM_080864.2
• UniProt Accession #: Q8WXF3; Q6UXW5
• Molecular Weight: 15,451 Da
• NCBI Official Full Name: relaxin-3 preproprotein
• NCBI Official Synonym Full Names: relaxin 3
• NCBI Official Symbol: RLN3
• NCBI Official Synonym Symbols: H3; RXN3; ZINS4; insl7
• NCBI Protein Information: relaxin-3; prorelaxin H3; insulin-like 7; insulin-like peptide 7; insulin-like peptide INSL7
• UniProt Protein Name: Relaxin-3
• Protein Family: Relaxin
• UniProt Gene Name: RLN3
• UniProt Synonym Gene Names: INSL7; RXN3; ZINS4
• UniProt Entry Name: REL3_HUMAN

NCBI Description

Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In the human there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3. RLN1 and RLN2 share high sequence homology. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. The protein encoded by this gene is a member of the relaxin family. The active form of the encoded protein consists of an A chain and a B chain but their cleavage sites are not definitely described yet. It may play a role in neuropeptide signaling processes. [provided by RefSeq]

Uniprot Description

RLN3: May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 (GPCR135) and relaxin-3 receptor-2 (GPCR142). Belongs to the insulin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular region

Molecular Function: protein binding; G-protein-coupled receptor binding; hormone activity