Mouse Rb1 Monoclonal Antibody | anti-Rb1 antibody

Rb1 (Tumor Suppressor Protein)

Cat. Num. AAA4380199

• Gene Names: RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
• Reactivity: Human, Mouse. Others not known.
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Synonyms: Rb1; Monoclonal Antibody; Rb1 (Tumor Suppressor Protein); OSRC; Osteosarcoma; p105-Rb; PP105; pp110; pRb; Prepro retinoblastoma associated protein; RB1; Retinoblastoma 1; Retinoblastoma-associated protein; anti-Rb1 antibody

• Host: Mouse
• Reactivity: Human, Mouse. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: SPM353
• Specificity: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 928

• Applicable Applications for anti-Rb1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) Formalin
• Application Notes: WB: 1-2 ug/ml; IP: 0.5-1ug/500ug protein lysate; IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.; ; Optimal dilution for a specific application should be determined.
• Immunogen: Recombinant human Rb1 protein fragment of 283 amino acid residues
• Cellular Localization: Nuclear
• Hu-chromosome Location: 13q14.2
• Positive Control: HeLa, A431 or BT-20 cells. Breast or Colon carcinoma.
• Preparation and Storage: Antibody with azide - store at 2 to 8°C.; Antibody without azide - store at -20 to -80°C.; Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (SPM353).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Melanoma stained with Bax Monoclonal Antibody (BAX/962).)

References

Grand et al. 1989. Oncogene. 4(11):1291-8. Bartek J, et. al. Oncogene 7:101-108, 1992.

NCBI and Uniprot Product Information

• NCBI GI #: 108773787
• NCBI GeneID: 5925
• NCBI Accession #: NP_000312.2
• NCBI GenBank Nucleotide #: NM_000321.2
• UniProt Accession #: P06400; P78499; Q5VW46; Q8IZL4; A8K5E3
• Molecular Weight: 105kDa
• NCBI Official Full Name: retinoblastoma-associated protein
• NCBI Official Synonym Full Names: RB transcriptional corepressor 1
• NCBI Official Symbol: RB1
• NCBI Official Synonym Symbols: RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
• NCBI Protein Information: retinoblastoma-associated protein
• UniProt Protein Name: Retinoblastoma-associated protein
• Protein Family: RB1-inducible coiled-coil protein
• UniProt Gene Name: RB1
• UniProt Synonym Gene Names: Rb

NCBI Description

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Uniprot Description

Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.