Mouse anti-Mouse, Human Rb1 Monoclonal Antibody | anti-Rb1 antibody
Mouse anti Rb1 [1F8]: Horseradish peroxidase (HRP)
Reactivity
Mouse, Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified on protein A from tissue culture supernatant.
Synonyms
Rb1; Monoclonal Antibody; Mouse anti Rb1 [1F8]: Horseradish peroxidase (HRP); OSRC; p105-Rb; pp110; pRb; Rb; RB1; retinoblastoma 1; retinoblastoma suspectibility protein; Retinoblastoma-associated protein; anti-Rb1 antibody
Host
Mouse
Reactivity
Mouse, Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1F8
Specificity
This antibody recognises retinoblastoma-associated protein (RB), a 105kDa tumour suppressor which is also known as Rb1. Rb1 functions as a transcription factor that controls cell cycle progression (at the G1/S phase transition) through its repression of E2F-mediated transcription. Mutation of the Rb gene is found frequently in human sarcomas, lung, bladder and breast carcinomas and is the molecular basis for hereditary disposition to retinoblastoma.
Purity/Purification
Purified on protein A from tissue culture supernatant.
Form/Format
Purified IgG conjugated to Horseradish Peroxidase, supplied in Phosphate buffered saline (PBS) containing 0.01% Thiomersal
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Rb1 antibody
Immunohistochemistry (IHC) Formalin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Optimal antibody dilution should be determined by titration
Target Antigen
Rb1
Immunogen
Rb-b-galactosidase fusion protein spanning nucleotides 1126-1973 of human Rb gel purified from bacterial lysates
Myeloma/fusion Partners
Cells from immunized Balb/c mice were fused with the Sp2/0-Ag.14 myeloma cell line
Research Areas
Epigenetics; Cell cycle; Tumour supressor
References
B¡rtek J, et al. Cellular localization and T antigen binding of the retinoblastoma protein. Oncogene.1992.7(1):101-8. PMID:1741157 http://www.ncbi.nlm.nih.gov/pubmed/1741157
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Product Notes
The Rb1 (Catalog #AAA375256) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Rb1 [1F8]: Horseradish peroxidase (HRP) reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Rb1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin, Immunoprecipitation (IP), Western Blot (WB). Optimal antibody dilution should be determined by titration. Researchers should empirically determine the suitability of the Rb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rb1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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