Rat Ras, H Monoclonal Antibody | anti-Ras85D antibody
Ras, H (Harvey Murine Sarcoma Virus Oncogene)
Gene Names
Ras85D; C-ras1; CG9375; D-Ras; D-ras-1; D-ras1; D-Ras1; Dm Ras1; DmelCG9375; Dmras85D; dRas; Dras; DRas; dras1; dRas1; dRAS1; Dras1; DRAS1; dRas85D; Dras85D; DRas85D/Ras; E(faf); E(sev)3C; EK3-4; fs(3)05703; l(3)06677; l(3)s1747; p21[Ras1]; ras; Ras; RAS; ras 1;
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Neutralization Assay
Purity
PurifiedPurified
Synonyms
Ras; H; Monoclonal Antibody; H (Harvey Murine Sarcoma Virus Oncogene); Anti -Ras; anti-Ras85D antibody
Host
Rat
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3G260
Specificity
Recognizes phosphorylated and non-phosphorylated human Ras. Species Crossreactivity: mouse and rat. Does not crossreact with the rap protein.
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Applicable Applications for anti-Ras85D antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Neutralization
Application Notes
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Neutralization.
Dilution: Immunofluorescence: 2.5ug/ml
Western Blot (chemiluminescence detection): 2.5ug/ml
Immunohistochemistry (Frozen): 5ug/ml
Immunohistochemistry: 1-5ug/ml. Staining of formalin fixed, paraffin embedded tissue requires pepsin pretreatment. Use saponin to permeablize.
Immunoprecipitation(autoradiographic detection): 1ug/sample Use rabbit anti-rat IgG plus Protein A or goat anti-rat IgG plus Protein G. Protein A alone will not immunoprecipitate rat IgG. Doublets corresponding to farnesylated- and non-farnesylated ras may be observed. Detects both phosphorylated and non-phosphorylated human ras as well as the p21 translational products of the H-, K- and N-ras human genes
Neutralization: Neutralizes H-, K- and N-ras p21 biochemical activity by binding to residues E63, S65, A66, M67, Q70 and R73.
Dilution: Immunofluorescence: 2.5ug/ml
Western Blot (chemiluminescence detection): 2.5ug/ml
Immunohistochemistry (Frozen): 5ug/ml
Immunohistochemistry: 1-5ug/ml. Staining of formalin fixed, paraffin embedded tissue requires pepsin pretreatment. Use saponin to permeablize.
Immunoprecipitation(autoradiographic detection): 1ug/sample Use rabbit anti-rat IgG plus Protein A or goat anti-rat IgG plus Protein G. Protein A alone will not immunoprecipitate rat IgG. Doublets corresponding to farnesylated- and non-farnesylated ras may be observed. Detects both phosphorylated and non-phosphorylated human ras as well as the p21 translational products of the H-, K- and N-ras human genes
Neutralization: Neutralizes H-, K- and N-ras p21 biochemical activity by binding to residues E63, S65, A66, M67, Q70 and R73.
Immunogen
Recombinant p21 ras protein. Epitope: aa62-76 of human ras.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Ras85D antibody
v-H-ras, K-ras and N-ras are 21kD phosphoproteins originally isolated based on their oncogenic properties and later found in all eukaryotic cells. Ras proteins belong to the Ras superfamily of monomeric GTPases which also includes the Rho, Rac, and Rab subfamilies. Ras proteins are anchored to the cytoplasmic face of plasma membranes where they function in switch-like fashion to relay signals from receptor tyrosine kinases to downstream effector molecules. Ras activation is regulated by the opposing effects of the activating guanine nucleotide exchange factors (GNEFs) that exchange ras-bound GDT with GTP and the inactivating GTPase accelerating proteins (GAPs). Ras activation of the MAP kinase signaling cascade is thought to occur via direct interaction of ras with the serine/thronine MAP-kinase-kinase-kinase raf.
Product Categories/Family for anti-Ras85D antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Ras oncogene at 85D
NCBI Official Synonym Full Names
Ras oncogene at 85D
NCBI Official Symbol
Ras85D
NCBI Official Synonym Symbols
C-ras1; CG9375; D-Ras; D-ras-1; D-ras1; D-Ras1; Dm Ras1; DmelCG9375; Dmras85D; dRas; Dras; DRas; dras1; dRas1; dRAS1; Dras1; DRAS1; dRas85D; Dras85D; DRas85D/Ras; E(faf); E(sev)3C; EK3-4; fs(3)05703; l(3)06677; l(3)s1747; p21[Ras1]; ras; Ras; RAS; ras 1;
NCBI Protein Information
CG9375 gene product from transcript CG9375-RA; CG9375-PA; Ras GTPase; Ras oncogene at 85B; Ras oncogene at 85D; Ras1; Ras85D-PA; enhancer of faf
Protein Family
Research Articles on Ras85D
Similar Products
Product Notes
The Ras85D (Catalog #AAA631630) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Ras, H (Harvey Murine Sarcoma Virus Oncogene) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ras, H can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Neutralization. Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Neutralization. Dilution: Immunofluorescence: 2.5ug/ml Western Blot (chemiluminescence detection): 2.5ug/ml Immunohistochemistry (Frozen): 5ug/ml Immunohistochemistry: 1-5ug/ml. Staining of formalin fixed, paraffin embedded tissue requires pepsin pretreatment. Use saponin to permeablize. Immunoprecipitation(autoradiographic detection): 1ug/sample Use rabbit anti-rat IgG plus Protein An or goat anti-rat IgG plus Protein G. Protein An alone will not immunoprecipitate rat IgG. Doublets corresponding to farnesylated- and non-farnesylated ras may be observed. Detects both phosphorylated and non-phosphorylated human ras as well as the p21 translational products of the H-, K- and N-ras human genes Neutralization: Neutralizes H-, K- and N-ras p21 biochemical activity by binding to residues E63, S65, A66, M67, Q70 and R73. Researchers should empirically determine the suitability of the Ras85D for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ras, H, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
