Western Blot (WB)
(The extracts of 293T (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500~1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cross-reacting of anti-human RAS (AT2G9) was analyzed by western blot with recombinant protein (200ng) of NRAS, KRAS and HRAS. )
Mouse anti-Human RAS Monoclonal Antibody | anti-RAS antibody
RAS antibody
Gene Names
NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
By protein-G affinity chromatography
Synonyms
RAS; Monoclonal Antibody; RAS antibody; Neuroblastoma RAS viral (v-ras) oncogene homolog; GTPase NRas; HRAS1; ALPS4; N-ras; NRAS1; NS6; Neuroblastoma RAS viral (v-ras) oncogene homolog OTTMuSP00000023521; AV095280; N ras; N ras protein part 4; Neuroblastoma RAS viral (v ras) oncogene homolog; OTTHuMP00000013879; Transforming protein N Ras; v ras neuroblastoma RAS viral oncogene homolog; anti-RAS antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT2G9
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
189
Applicable Applications for anti-RAS antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500 ~ 1:5000.
Recommended starting dilution is 1:1000
Recommended starting dilution is 1:1000
Antigen Species
Human
Immunogen
Recombinant human NRAS (1-186aa) purified from E. coli
Clone Note
Anti-human RAS mAb, clone AT2G9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NRAS protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The extracts of 293T (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500~1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cross-reacting of anti-human RAS (AT2G9) was analyzed by western blot with recombinant protein (200ng) of NRAS, KRAS and HRAS. )
Western Blot (WB)
(The extracts of 293T (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500~1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cross-reacting of anti-human RAS (AT2G9) was analyzed by western blot with recombinant protein (200ng) of NRAS, KRAS and HRAS. )
Immunofluorescence (IF)
(ICC/IF analysis of RAS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RAS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of RAS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RAS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-RAS antibody
The Ras protein is a subfamily of small GTPases that are involved in signal transduction. They are activated by ligand-binding receptor tyrosine kinases such as EGFR, PDGFR, CSF and FGF. These kinases transiently convert RAS-GDP (inactive form) to RAS-GTP, the active form of RAS. The main genes are HRAS, NRAS and KRAS.
Product Categories/Family for anti-RAS antibody
References
Rocks O, et al. (2006). Current Opinion in Cell Biology 18 (4): 351-7.; Wennerberg K, et al. (March 2005). J. Cell. Sci. 118 (Pt 5): 843-6.; Rotblat B, et al. (2008). Methods Enzymol 439: 467-89.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,229 Da
NCBI Official Full Name
GTPase NRas
NCBI Official Synonym Full Names
NRAS proto-oncogene, GTPase
NCBI Official Symbol
NRAS
NCBI Official Synonym Symbols
NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
NCBI Protein Information
GTPase NRas
UniProt Protein Name
GTPase NRas
Protein Family
UniProt Gene Name
NRAS
UniProt Synonym Gene Names
HRAS1
NCBI Description
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]
Uniprot Description
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Research Articles on RAS
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Product Notes
The RAS nras (Catalog #AAA200206) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAS antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAS can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:500 ~ 1:5000. Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the RAS nras for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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