Mouse anti-Human RAPGEF6 Monoclonal Antibody | anti-RAPGEF6 antibody

Mouse Monoclonal [clone 2C5] (IgG2a,k) to Human RAPGEF6

Cat. Num. AAA245856

• Gene Names: RAPGEF6; RAGEF2; PDZGEF2; KIA001LB; PDZ-GEF2; RA-GEF-2
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: RAPGEF6; Monoclonal Antibody; Mouse Monoclonal [clone 2C5] (IgG2a; k) to Human RAPGEF6; Anti-RAPGEF6 Antibody (clone 2C5) IHC-plus; PDZGEF2; KIA001LB; RA-GEF-2; PDZ-GEF2; RAGEF2; Human RAPGEF6; anti-RAPGEF6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2C5
• Specificity: Human RAPGEF6
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 180915260

• Applicable Applications for anti-RAPGEF6 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (40 ug/ml), IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot using cell line lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: RAPGEF6 (NP_057424, 1012 a.a. ~ 1111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: RAPGEF6 antibody was raised against rAPGEF6 (NP_057424, 1012 a.a. ~ 1111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-RAPGEF6 / RAGEF2 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to RAPGEF6 on HeLa cell. [antibody concentration 40 ug/ml])

Western Blot (WB)

(in Western blot of RAPGEF6 expression in HeLa NE.)

NCBI and Uniprot Product Information

• NCBI GI #: 224589817
• NCBI GeneID: 51735
• NCBI Accession #: NC_000005.9
• NCBI GenBank Nucleotide #: NC_000005.9
• Molecular Weight: 93,442 Da
• NCBI Official Full Name: Homo sapiens chromosome 5, GRCh37.p13 Primary Assembly
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor (GEF) 6
• NCBI Official Symbol: RAPGEF6
• NCBI Official Synonym Symbols: RAGEF2; PDZGEF2; KIA001LB; PDZ-GEF2; RA-GEF-2
• NCBI Protein Information: rap guanine nucleotide exchange factor 6; PDZ domain-containing guanine nucleotide exchange factor 2; PDZ domain-containing guanine nucleotide exchange factor I; PDZ domain containing guanine nucleotide exchange factor (GEF) 2
• UniProt Protein Name: Rap guanine nucleotide exchange factor 6
• Protein Family: Rap guanine nucleotide exchange factor
• UniProt Gene Name: RAPGEF6
• UniProt Synonym Gene Names: PDZGEF2; PDZ-GEF2
• UniProt Entry Name: RPGF6_HUMAN

Uniprot Description

RAPGEF6: Guanine nucleotide exchange factor (GEF) for Rap1A, Rap2A and M-Ras GTPases. Does not interact with cAMP. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Ras; GEFs

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: endocytic vesicle; apical plasma membrane; plasma membrane; cytosol

Molecular Function: protein binding; Ras GTPase binding; GTP-dependent protein binding; guanyl-nucleotide exchange factor activity

Biological Process: microvillus biogenesis; regulation of GTPase activity; Ras protein signal transduction