Western Blot (WB)
(RAP2B monoclonal antibody, Western Blot analysis of RAP2B expression in A-431.)
Mouse anti-Human RAP2B Monoclonal Antibody | anti-RAP2B antibody
RAP2B (Ras-related Protein Rap-2b, MGC20484) (AP)
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RAP2B; Monoclonal Antibody; RAP2B (Ras-related Protein Rap-2b; MGC20484) (AP); anti-RAP2B antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
4F12-3C6
Specificity
Recognizes human RAP2B.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-RAP2B antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to human RAP2B, aa1-184 (AAH12362) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MREYKVVVLGSGGVGKSALTVQFVTGSFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYERVPMILVGNKVDLEGEREVSYGEGKALAEEWSCPFMETSAKNKASVDELFAEIVRQMNYAAQPNGDEGCCSACVIL
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(RAP2B monoclonal antibody, Western Blot analysis of RAP2B expression in A-431.)
Western Blot (WB)
(RAP2B monoclonal antibody, Western Blot analysis of RAP2B expression in A-431.)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to RAP2B on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 1ug/ml])
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to RAP2B on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 1ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to RAP2B on HeLa cell. [antibody concentration 10ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to RAP2B on HeLa cell. [antibody concentration 10ug/ml])
Product Categories/Family for anti-RAP2B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
20,504 Da
NCBI Official Full Name
Homo sapiens RAP2B, member of RAS oncogene family, mRNA
NCBI Official Synonym Full Names
RAP2B, member of RAS oncogene family
NCBI Official Symbol
RAP2B
NCBI Protein Information
ras-related protein Rap-2b
Protein Family
NCBI Description
This intronless gene belongs to a family of RAS-related genes. The proteins encoded by these genes share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Evidence suggests that this protein may be polyisoprenylated and palmitoylated. [provided by RefSeq, Jul 2008]
Research Articles on RAP2B
Similar Products
Product Notes
The RAP2B (Catalog #AAA6133312) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAP2B (Ras-related Protein Rap-2b, MGC20484) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAP2B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RAP2B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAP2B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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