Western Blot (WB)
(QARS monoclonal antibody Western Blot analysis of QARS expression in HeLa.)
Mouse anti-Human QARS Monoclonal Antibody | anti-QARS antibody
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
Western Blot (WB)
(QARS monoclonal antibody Western Blot analysis of QARS expression in HeLa.)
Western Blot (WB)
(QARS monoclonal antibody Western Blot analysis of QARS expression in HeLa.)
Western Blot (WB)
(Western Blot analysis of QARS expression in transfected 293T cell line by QARS monoclonal antibody.|Lane 1: QARS transfected lysate (87.8kD).|Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of QARS expression in transfected 293T cell line by QARS monoclonal antibody.|Lane 1: QARS transfected lysate (87.8kD).|Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to QARS on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to QARS on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])
Immunoprecipitation (IP)
(Immunoprecipitation of QARS transfected lysate using QARS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with QARS rabbit polyclonal antibody.)
Immunoprecipitation (IP)
(Immunoprecipitation of QARS transfected lysate using QARS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with QARS rabbit polyclonal antibody.)
Testing Data
(Detection limit for recombinant GST tagged QARS is ~1ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged QARS is ~1ng/ml as a capture antibody.)
NCBI and Uniprot Product Information
NCBI Description
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
Uniprot Description
QARS: catalyzes the formation of L-glutaminyl-tRNA. Belongs to the class-I aminoacyl-tRNA synthetase family.
Protein type: Translation; Ligase; Mitochondrial; EC 6.1.1.18
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: mitochondrial matrix; cytoplasm; cytosol
Molecular Function: protein binding; glutamine-tRNA ligase activity; ATP binding
Biological Process: tRNA aminoacylation for protein translation; glutaminyl-tRNA aminoacylation; gene expression; brain development
Disease: Microcephaly, Progressive, With Seizures And Cerebral And Cerebellar Atrophy
Similar Products
Product Notes
The QARS qars (Catalog #AAA6012851) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's QARS can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml. Researchers should empirically determine the suitability of the QARS qars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: FIHWVSQPLM CEVRLYERLF QHKNPEDPTE VPGGFLSDLN LASLHVVDAA LVDCSVALAK PFDKFQFERL GYFSVDPDSH QGKLVFNRTV TLKEDPGKV. It is sometimes possible for the material contained within the vial of "QARS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
