Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PYCRL protein (Right) or empty vector (Left) detected with PYCRL antibody)
Mouse PYCRL Monoclonal Antibody | anti-PYCRL antibody
PYCRL antibody
Gene Names
PYCRL; PYCR3
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
PYCRL antibody was purified by affinity chromatography.
Synonyms
PYCRL; Monoclonal Antibody; PYCRL antibody; Monoclonal PYCRL; Anti-PYCRL; Pyrroline 5 carboxylate reductase like; FLJ13852; P5C reductase 3; P5CR 3; OTTHUMP00000211293; anti-PYCRL antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1B12
Purity/Purification
PYCRL antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
274
Applicable Applications for anti-PYCRL antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
IHC: 1:150
WB: 1:500-2000
IF: 1:100
IHC: 1:150
WB: 1:500-2000
Biological Significance
PYCRL belongs to the pyrroline-5-carboxylate reductase family. The function of the PYCRL protein is not known.
Cross-Reactivity
Human, Rat
Immunogen
PYCRL antibody was raised in mouse using a full length recombinant protein of human PYCRL (NP_075566) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PYCRL protein (Right) or empty vector (Left) detected with PYCRL antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PYCRL protein (Right) or empty vector (Left) detected with PYCRL antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PYCRL protein in paraffin embedded Carcinoma of Human kidney tissue using PYCRL antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PYCRL protein in paraffin embedded Carcinoma of Human kidney tissue using PYCRL antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PYCRL protein using PYCRL antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PYCRL protein using PYCRL antibody)
Related Product Information for anti-PYCRL antibody
Mouse monoclonal PYCRL antibody
Product Categories/Family for anti-PYCRL antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
26,440 Da
NCBI Official Full Name
PYCRL, partial
NCBI Official Synonym Full Names
pyrroline-5-carboxylate reductase-like
NCBI Official Symbol
PYCRL
NCBI Official Synonym Symbols
PYCR3
NCBI Protein Information
pyrroline-5-carboxylate reductase 3
UniProt Protein Name
Pyrroline-5-carboxylate reductase 3
UniProt Gene Name
PYCRL
UniProt Synonym Gene Names
P5C reductase 3; P5CR 3
UniProt Entry Name
P5CR3_HUMAN
Uniprot Description
PYCRL: Belongs to the pyrroline-5-carboxylate reductase family.
Protein type: EC 1.5.1.2; Oxidoreductase; Amino Acid Metabolism - arginine and proline; Hydrolase
Chromosomal Location of Human Ortholog: 8q24.3
Molecular Function: pyrroline-5-carboxylate reductase activity
Similar Products
Product Notes
The PYCRL pycrl (Catalog #AAA838216) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PYCRL can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC: 1:100 IF: 1:100 IHC: 1:150 WB: 1:500-2000. Researchers should empirically determine the suitability of the PYCRL pycrl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PYCRL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
