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Western Blot (WB) (Western blot analysis of PSME1 on MCF-7 cell (1) and rat lung tissue (2) lysate using anti-PSME1 antibody at 1/500 dilution.)

Rabbit PSME1 Monoclonal Antibody | anti-PSME1 antibody

PSME1 Antibody

Gene Names
PSME1; PA28A; IFI5111; REGalpha; PA28alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
PSME1; Monoclonal Antibody; PSME1 Antibody; 11S regulator complex alpha subunit antibody; 11S regulator complex subunit alpha antibody; 29 kD MCP activator subunit antibody; 29kD MCP activator subunit antibody; Activator of multicatalytic protease subunit 1 antibody; IFI5111 antibody; IGUP I-5111 antibody; Interferon gamma IEF SSP 5111 antibody; Interferon gamma inducible protein 5111 antibody; Interferon gamma up regulated I 5111 protein antibody; Interferon gamma up-regulated I-5111 protein antibody; MGC8628 antibody; PA28a antibody; PA28alpha antibody; Proteasome (prosome; macropain) activator subunit 1 (PA28 alpha) antibody; Proteasome activator 28 subunit alpha antibody; Proteasome activator complex subunit 1 antibody; Proteasome activator subunit 1 antibody; PSME1 antibody; PSME1_HUMAN antibody; REG-alpha antibody; REGalpha antibody; anti-PSME1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JU60-33
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PSME1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:100
IP: 1:10-1:50
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of PSME1 on MCF-7 cell (1) and rat lung tissue (2) lysate using anti-PSME1 antibody at 1/500 dilution.)

Western Blot (WB) (Western blot analysis of PSME1 on MCF-7 cell (1) and rat lung tissue (2) lysate using anti-PSME1 antibody at 1/500 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PSME1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PSME1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PSME1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PSME1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PSME1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PSME1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-PSME1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-PSME1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PSME1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PSME1 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining PSME1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining PSME1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining PSME1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining PSME1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining PSME1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining PSME1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-PSME1 antibody
Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
Product Categories/Family for anti-PSME1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,870 Da
NCBI Official Full Name
proteasome activator complex subunit 1 isoform 3
NCBI Official Synonym Full Names
proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
NCBI Official Symbol
PSME1
NCBI Official Synonym Symbols
PA28A; IFI5111; REGalpha; PA28alpha
NCBI Protein Information
proteasome activator complex subunit 1; 11S regulator complex subunit alpha; 29-kD MCP activator subunit; IGUP I-5111; activator of multicatalytic protease subunit 1; interferon gamma up-regulated I-5111 protein; interferon-gamma IEF SSP 5111; interferon-
UniProt Protein Name
Proteasome activator complex subunit 1
UniProt Gene Name
PSME1
UniProt Synonym Gene Names
IFI5111; REG-alpha; IGUP I-5111; PA28a; PA28alpha
UniProt Entry Name
PSME1_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

PSME1: Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. Heterodimer of PSME1 and PSME2, which forms a hexadimeric ring. PSME1 can form homoheptamers. By IFNG/IFN-gamma. Belongs to the PA28 family.

Protein type: Proteasome complex

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; proteasome complex; proteasome activator complex; cytoplasm; cytosol

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis

Research Articles on PSME1

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Product Notes

The PSME1 psme1 (Catalog #AAA9436522) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PSME1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PSME1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC: 1:50-1:100 IP: 1:10-1:50. Researchers should empirically determine the suitability of the PSME1 psme1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSME1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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