Mouse anti-Human PSMA7 Monoclonal Antibody | anti-PSMA7 antibody

Anti-PSMA7 Antibody (clone 1A10-3G12)

Cat. Num. AAA2400899

• Gene Names: PSMA7; C6; HSPC; RC6-1; XAPC7; HEL-S-276
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified from ascites by Protein A
• Synonyms: PSMA7; Monoclonal Antibody; Anti-PSMA7 Antibody (clone 1A10-3G12); Mouse Monoclonal [clone 3F11] (IgG1) to Human PSMA7; Proteasome subunit RC6-1; XAPC7; Proteasome subunit alpha 4; Proteasome subunit XAPC7; RC6-1; HSPC; anti-PSMA7 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, k
• Clone Number: 1A10-3G12
• Specificity: Human PSMA7
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4.
• Sequence Length: 248

• Applicable Applications for anti-PSMA7 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IF: 1-10 ug/ml; IHC-P: 20 ug/ml
• Immunogen: PSMA7 (AAH04427, 1 a.a. ~ 249 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C or lower.; Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PSMA7/HSPC antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to PSMA7 on HeLa cell. [antibody concentration 1 ~10 ug/ml].)

Western Blot (WB)

(PSMA7 monoclonal antibody (M01), clone 1A10-3G12. Western blot of PSMA7 expression in human omentum, serous carcinoma.)

Western Blot (WB)

(PSMA7 monoclonal antibody (M01), clone 1A10-3G12 Western blot of PSMA7 expression in HeLa.)

Western Blot (WB)

(Western blot of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody (M01), clone 1A10-3G12.)

Immunoprecipitation (IP)

(Immunoprecipitation of PSMA7 transfected lysate using anti-PSMA7 monoclonal antibody and Protein A Magnetic Bead.)

ELISA (EIA)

(Detection limit for recombinant GST tagged PSMA7 is approximately 1 ng/ml as a capture antibody.)

Related Product Information for anti-PSMA7 antibody

The proteasome is a multicatalytic protein ase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Plays animportant role in the regulation of cell proliferation or cell cyclecontrol, transcriptional regulation, immune and stress response, celldifferentiation, and apoptosis.

Product Categories/Family for anti-PSMA7 antibody

Protease Non Catalytic; Threonine T1

NCBI and Uniprot Product Information

• NCBI GI #: 4506189
• NCBI GeneID: 5688
• NCBI Accession #: NP_002783.1
• NCBI GenBank Nucleotide #: NM_002792.3
• UniProt Accession #: O14818; Q5JXJ2; Q9BR53; Q9H4K5; Q9UEU8; B2R515
• Molecular Weight: 16,135 Da
• NCBI Official Full Name: proteasome subunit alpha type-7
• NCBI Official Synonym Full Names: proteasome subunit alpha 7
• NCBI Official Symbol: PSMA7
• NCBI Official Synonym Symbols: C6; HSPC; RC6-1; XAPC7; HEL-S-276
• NCBI Protein Information: proteasome subunit alpha type-7
• UniProt Protein Name: Proteasome subunit alpha type-7
• Protein Family: Proteasome
• UniProt Gene Name: PSMA7
• UniProt Synonym Gene Names: HSPC

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the peptidase T1A family that functions as a 20S core alpha subunit. The encoded protein interacts with the hepatitis B virus X protein and plays a role in regulating hepatitis C virus internal ribosome entry site (IRES) activity, an activity essential for viral replication. The encoded protein also plays a role in the cellular stress response by regulating hypoxia-inducible factor-1alpha. A pseudogene of this gene is located on the long arm of chromosome 9. [provided by RefSeq, Jul 2012]

Uniprot Description

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions. The interaction with EMAP2 increases the proteasome-mediated HIF-1A degradation under the hypoxic conditions. Plays a role in hepatitis C virus internal ribosome entry site-mediated translation. Mediates nuclear translocation of the androgen receptor (AR) and thereby enhances androgen-mediated transactivation. Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response.