Flow Cytometry (FC/FACS)
(Figure 1. 1e5 of Expi 293 cell line transfected with human PSCA were stained with 100 uL of 1:100 diluted Biotinylated Anti-PSCA antibody (DM87), Rabbit mAb (Red histogram) or isotype control (Blue histogram) respectively, washed and then stained with Streptavidin APC. The experimental samples were analyzed by flow cytometry.)
Rabbit anti-Human PSCA Monoclonal Antibody | anti-PSCA antibody
Biotinylated Anti-PSCA antibody (DM87), Rabbit mAb
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Purified from cell culture supernatant by affinity chromatography
Synonyms
PSCA; Monoclonal Antibody; Biotinylated Anti-PSCA antibody (DM87); Rabbit mAb; UNQ206; PRO232; anti-PSCA antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
DM87
Purity/Purification
Purified from cell culture supernatant by affinity chromatography
Form/Format
Liquid. 50% Glycerol; PBS, pH7.4; 0.1% BSA. 0.1% Procline 300
Applicable Applications for anti-PSCA antibody
ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
ELISA: 1:5000-10,000
FC/FACS: 1:100
FC/FACS: 1:100
Immunogen
Recombinant human PSCA (Leu12-Ser86) produced by using human HEK293 cells
Reconstitution
Reconstitute with deionized water
Preparation and Storage
Store at -20 degree C for 12 months (Avoid repeated freezing and thawing)
Flow Cytometry (FC/FACS)
(Figure 1. 1e5 of Expi 293 cell line transfected with human PSCA were stained with 100 uL of 1:100 diluted Biotinylated Anti-PSCA antibody (DM87), Rabbit mAb (Red histogram) or isotype control (Blue histogram) respectively, washed and then stained with Streptavidin APC. The experimental samples were analyzed by flow cytometry.)
Flow Cytometry (FC/FACS)
(Figure 1. 1e5 of Expi 293 cell line transfected with human PSCA were stained with 100 uL of 1:100 diluted Biotinylated Anti-PSCA antibody (DM87), Rabbit mAb (Red histogram) or isotype control (Blue histogram) respectively, washed and then stained with Streptavidin APC. The experimental samples were analyzed by flow cytometry.)
Related Product Information for anti-PSCA antibody
This gene encodes a glycosylphosphatidylinositol-anchored cell membrane glycoprotein. In addition to being highly expressed in the prostate it is also expressed in the bladder, placenta, colon, kidney, and stomach. This gene is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. This gene includes a polymorphism that results in an upstream start codon in some individuals; this polymorphism is thought to be associated with a risk for certain gastric and bladder cancers. Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
12,912 Da
NCBI Official Full Name
Prostate stem cell antigen
UniProt Protein Name
Prostate stem cell antigen
Protein Family
UniProt Gene Name
PSCA
UniProt Entry Name
PSCA_HUMAN
Uniprot Description
PSCA: May be involved in the regulation of cell proliferation. Has a cell-proliferation inhibition activity in vitro.
Protein type: Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 8q24.2
Cellular Component: plasma membrane
Similar Products
Product Notes
The PSCA psca (Catalog #AAA188710) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Biotinylated Anti-PSCA antibody (DM87), Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSCA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:5000-10,000 FC/FACS: 1:100. Researchers should empirically determine the suitability of the PSCA psca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSCA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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