Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa Protocadherin Gamma protein using Mouse Anti-Protocadherin Gamma Monoclonal Antibody, Clone S159-5. Load: 10 ug. Primary Antibody: Mouse Anti-Protocadherin Gamma Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.)
Mouse anti-Mouse, Rat Protocadherin Gamma Monoclonal Antibody | anti-Pcdhga1 antibody
Protocadherin Gamma (pan) Antibody: HRP
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Synonyms
Protocadherin Gamma; Monoclonal Antibody; Protocadherin Gamma (pan) Antibody: HRP; PCDH; Protocadherin Gamma antibody; PCDH gamma antibody; PCDH-gamma; PCDHG; Protocadherin gamma; Gamma Protocadherin (pan); Pan-Gamma-Protocadherin; Pan Gamma Protocadherin; anti-Pcdhga1 antibody
Host
Mouse
Reactivity
Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S159-5
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
931
Applicable Applications for anti-Pcdhga1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
1:1000 WB
Conjugate
HRP
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Preparation and Storage
-20 degree C
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa Protocadherin Gamma protein using Mouse Anti-Protocadherin Gamma Monoclonal Antibody, Clone S159-5. Load: 10 ug. Primary Antibody: Mouse Anti-Protocadherin Gamma Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.)
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa Protocadherin Gamma protein using Mouse Anti-Protocadherin Gamma Monoclonal Antibody, Clone S159-5. Load: 10 ug. Primary Antibody: Mouse Anti-Protocadherin Gamma Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.)
Related Product Information for anti-Pcdhga1 antibody
Background Info: Detects a 100kDa protein. Cross-reacts with all Gamma- protocadherin-A, -B and -C proteins.
Scientific Background: The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
Scientific Background: The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
Product Categories/Family for anti-Pcdhga1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
101,367 Da
NCBI Official Full Name
protocadherin gamma-A1
NCBI Official Synonym Full Names
protocadherin gamma subfamily A, 1
NCBI Official Symbol
Pcdhga1
NCBI Protein Information
protocadherin gamma-A1
UniProt Protein Name
MCG133388, isoform CRA_t
Protein Family
UniProt Gene Name
Pcdhga1
UniProt Synonym Gene Names
mCG_133388
UniProt Entry Name
Q91XZ0_MOUSE
Uniprot Description
PCDHGA1: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Calcium-binding; Membrane protein, integral; Cell adhesion
Cellular Component: membrane
Molecular Function: protein binding
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Product Notes
The Pcdhga1 pcdhga1 (Catalog #AAA803817) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Protocadherin Gamma (pan) Antibody: HRP reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Protocadherin Gamma can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). 1:1000 WB. Researchers should empirically determine the suitability of the Pcdhga1 pcdhga1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protocadherin Gamma, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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