Mouse anti-Human Prostate Specific Antigen Monoclonal Antibody | anti-PSA antibody

Prostate Specific Antigen (PSA, Prostate-specific, APS, Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3, KLK3, KLK2A1, P-30 antigen, Semenogelase, Seminin) (HRP)

Cat. Num. AAA6124478

• Gene Names: KLK3; APS; PSA; hK3; KLK2A1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: Prostate Specific Antigen; Monoclonal Antibody; Prostate Specific Antigen (PSA; Prostate-specific; APS; Gamma-seminoprotein; hK3; Kallikrein related peptidase 3; Kallikrein-3; KLK3; KLK2A1; P-30 antigen; Semenogelase; Seminin) (HRP); PSA; Seminin; anti-PSA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: SPM352
• Specificity: Recognizes human Prostate Specific Antigen.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-PSA antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 0.5-1ug/500ug protein lysate; WB: 0.5-1.0ug/ml; IHC: 0.5-1.0ug/ml for 30 minutes at RT; Applications are based on unconjugated antibody.
• Immunogen: PSA from human sperm plasma. Cellular Localization: Cytoplasmic
• Conjugate: HRP
• Positive Control: PC12 cells or normal prostate or prostate carcinoma
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded prostate carcinoma stained with PSA MAb (SPM352).)

Product Categories/Family for anti-PSA antibody

Antibodies; Cancer Markers

References

1. ISOBM TD-3 Workshop, Tum. Biol. 20 (S1), pp 1-94, Eds. Rye P.D. et al. (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 71834853
• NCBI GeneID: 354
• NCBI Accession #: NP_001025218.1
• NCBI GenBank Nucleotide #: NM_001030047.1
• UniProt Accession #: P07288; Q15096; Q16272; Q86TG8; C9JXH3; G3V0H4; G3XAE3
• Molecular Weight: 28,741 Da
• NCBI Official Full Name: prostate-specific antigen isoform 3 preproprotein
• NCBI Official Synonym Full Names: kallikrein-related peptidase 3
• NCBI Official Symbol: KLK3
• NCBI Official Synonym Symbols: APS; PSA; hK3; KLK2A1
• NCBI Protein Information: prostate-specific antigen; seminin; P-30 antigen; kallikrein-3; semenogelase; gamma-seminoprotein; prostate specific antigen
• UniProt Protein Name: Prostate-specific antigen
• UniProt Gene Name: KLK3
• UniProt Synonym Gene Names: APS; PSA; Seminin
• UniProt Entry Name: KLK3_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: Secreted; Protease; EC 3.4.21.77; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region; nucleus

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of angiogenesis; cellular protein metabolic process; proteolysis