Mouse Proliferating Cell Nuclear Monoclonal Antibody | anti-PCNA antibody
Mouse Anti Human Proliferating Cell Nuclear Antigen
Applications
ELISA, Western Blot
Purity
PCNA antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Synonyms
Proliferating Cell Nuclear; Monoclonal Antibody; Mouse Anti Human Proliferating Cell Nuclear Antigen; PCNA Antibody; Proliferating Cell Nuclear Antigen; Mouse Anti Human; Proliferating cell nuclear antigen; PCNA; Cyclin; MGC8367; anti-PCNA antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG3 heavy chain and kappa light chain
Clone Number
Pk1A3AT
Purity/Purification
PCNA antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Form/Format
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Sequence Length
261
Applicable Applications for anti-PCNA antibody
ELISA (EIA), Western Blot (WB)
Application Notes
PCNA antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 3,000. Recommended starting dilution is 1:2,000.
Immunogen
Anti-human PCNA mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PCNA amino acids 1-261 purified from E Coli.
Preparation and Storage
For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.
12 months at -20 degree C. 1 month at 4 degree C.
12 months at -20 degree C. 1 month at 4 degree C.
Related Product Information for anti-PCNA antibody
Introduction: PCNA is located in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps elevate the processivity of leading strand synthesis during DNA replication. In reaction to DNA damage, PCNA is ubiquitinated and takes part in the RAD6-dependent DNA repair pathway. 2 transcript variants encoding the same protein have been found for PCNA gene. Pseudogenes of this PCNA gene have been described on chromosome 4 and on the X chromosome. PCNA is expressed during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA is induced by UV irradiation.
Product Categories/Family for anti-PCNA antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
28,706 Da
NCBI Official Full Name
Proliferating cell nuclear antigen (PCNA)
UniProt Protein Name
Proliferating cell nuclear antigen
Protein Family
UniProt Gene Name
PCNA
UniProt Entry Name
Q6FHF5_HUMAN
Uniprot Description
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.
Similar Products
Product Notes
The PCNA pcna (Catalog #AAA140300) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Proliferating Cell Nuclear can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). PCNA antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 3,000. Recommended starting dilution is 1:2,000. Researchers should empirically determine the suitability of the PCNA pcna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Proliferating Cell Nuclear, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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