Mouse anti-Human PRKDC / DNA-PKcs Monoclonal Antibody | anti-PRKDC antibody

Mouse Monoclonal [clone 1B9] (IgG) to Human PRKDC / DNA-PKcs

Cat. Num. AAA245753

• Gene Names: PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: PRKDC / DNA-PKcs; Monoclonal Antibody; Mouse Monoclonal [clone 1B9] (IgG) to Human PRKDC / DNA-PKcs; Anti-PRKDC / DNA-PKcs Antibody (clone 1B9) IHC-plus; PRKDC; DNA-PK; DNAPK; DNPK1; HYRC; HYRC1; p350; Scid; XRCC7; DNA-PK catalytic subunit; DNA-PKcs; p460; Human PRKDC; anti-PRKDC antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 1B9
• Specificity: Human DNA PKcs
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 4128

• Applicable Applications for anti-PRKDC antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western blot using cell line lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: PRKDC (NP_008835, 4019 a.a. ~ 4129 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: PRKDC / DNA-PKcs antibody was raised against pRKDC (NP_008835, 4019 a.a. ~ 4129 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-DNA-PKcs antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(PRKDC monoclonal antibody clone 1B9 Western blot of PRKDC expression in A-431.)

ELISA (EIA)

(Detection limit for recombinant GST tagged PRKDC is 1 ng/ml as a capture antibody.)

Product Categories/Family for anti-PRKDC antibody

Family: Protein Kinase; Subfamily: PI3/PI4

NCBI and Uniprot Product Information

• NCBI GI #: 13654237
• NCBI GeneID: 5591
• NCBI Accession #: NP_008835.5
• NCBI GenBank Nucleotide #: NM_006904.6
• UniProt Accession #: P78527; P78528; Q13327; Q13337; Q14175; Q59H99; Q7Z611; Q96SE6; Q9UME3
• Molecular Weight: 465,501 Da
• NCBI Official Full Name: DNA-dependent protein kinase catalytic subunit isoform 1
• NCBI Official Synonym Full Names: protein kinase, DNA-activated, catalytic polypeptide
• NCBI Official Symbol: PRKDC
• NCBI Official Synonym Symbols: HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs
• NCBI Protein Information: DNA-dependent protein kinase catalytic subunit; p460; DNA-PK catalytic subunit; hyper-radiosensitivity of murine scid mutation, complementing 1
• UniProt Protein Name: DNA-dependent protein kinase catalytic subunit
• UniProt Gene Name: PRKDC
• UniProt Synonym Gene Names: HYRC; HYRC1; DNA-PK catalytic subunit; DNA-PKcs
• UniProt Entry Name: PRKDC_HUMAN

NCBI Description

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]

Uniprot Description

DNAPK: an atypical protein kinase of the PIKK family. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. and modulation of transcription. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Defects cause severe combined immune deficiency (SCID) which is characterized by a lack of mature functional lymphocytes and a high susceptibility to lethal opportunistic infections. Required for repair of radiation-induced dsDNA breaks. Loss of function in mice or horses leads to the SCID (severe combined immune deficiency) phenotype due to failure of immunoglobulin rearrangement. Target of mutation in mismatch repair-deficient colorectal cancer. Inhibitor: KU-7059. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; EC 2.7.11.1; DNA repair, damage; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; Kinase, protein; ATYPICAL group; PIKK family; DNAPK subfamily

Chromosomal Location of Human Ortholog: 8q11

Cellular Component: nucleoplasm; transcription factor complex; membrane; nucleolus; DNA-dependent protein kinase complex; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; enzyme binding; DNA binding; DNA-dependent protein kinase activity; transcription factor binding; ATP binding; protein kinase activity

Biological Process: positive regulation of apoptosis; heart development; germ cell programmed cell death; rhythmic process; T cell differentiation in the thymus; double-strand break repair via nonhomologous end joining; negative regulation of protein amino acid phosphorylation; double-strand break repair; positive regulation of interferon type I production; response to gamma radiation; telomere maintenance; pro-B cell differentiation; somitogenesis; protein destabilization; immunoglobulin V(D)J recombination; B cell lineage commitment; protein modification process; regulation of circadian rhythm; DNA repair; double-strand break repair via homologous recombination; T cell lineage commitment; peptidyl-serine phosphorylation; DNA damage response, signal transduction resulting in induction of apoptosis; cellular response to insulin stimulus; T cell receptor V(D)J recombination; innate immune response; positive regulation of transcription from RNA polymerase II promoter; brain development

Disease: Immunodeficiency 26 With Or Without Neurologic Abnormalities