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Western Blot (WB)
(Western blot analysis of PRDM2 in PRDM2-transgenic lysate. HRP-conjugated anti-mouse secondary antibody/chemiluminescence detection were used for signal visualization.)
Mouse anti-Human, Mouse PRDM2 Monoclonal Antibody | anti-Prdm2 antibody
PRDM2 (RIZ1) (PRDM2, KMT8, RIZ, PR domain zinc finger protein 2, GATA-3-binding protein G3B, Lysine N-methyltransferase 8, MTB-ZF, MTE-binding protein, PR domain-containing protein 2, Retinoblastoma protein-interacting zinc finger protein, Zinc finger pro
Gene Names
Prdm2; Riz; KMT8; Riz1; Gm1033; Gm19732; Znfpr1c1; 4833427P12Rik; E330024L24Rik
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
PRDM2; Monoclonal Antibody; PRDM2 (RIZ1) (PRDM2; KMT8; RIZ; PR domain zinc finger protein 2; GATA-3-binding protein G3B; Lysine N-methyltransferase 8; MTB-ZF; MTE-binding protein; PR domain-containing protein 2; Retinoblastoma protein-interacting zinc finger protein; Zinc finger pro; Anti -PRDM2 (RIZ1) (PRDM2; anti-Prdm2 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
33AT1045
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Applicable Applications for anti-Prdm2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot, Immunoprecipitation, ELISA.
Dilution: ELISA: 1:1,000
Western Blot: 1:100~500
Immuno precipitation
Dilution: ELISA: 1:1,000
Western Blot: 1:100~500
Immuno precipitation
Immunogen
Purified recombinant GST fusion protein encoding aa 1-347 of human RIZ1. antibody is specific for RIZ1, it does not recognize the isoform beta (RIZ2).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of PRDM2 in PRDM2-transgenic lysate. HRP-conjugated anti-mouse secondary antibody/chemiluminescence detection were used for signal visualization.)
Western Blot (WB)
(Western blot analysis of PRDM2 in PRDM2-transgenic lysate. HRP-conjugated anti-mouse secondary antibody/chemiluminescence detection were used for signal visualization.)
Immunoprecipitation (IP)
(ChIP analysis of estrogen target genes. (A) Soluble chromatin was prepared from MCF7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed using antibody MBS643679. DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter. Adapted from Fig 6 in: Carling et al., 2004. Mol Cell Bio. 24 (16): 7032-7042.)
Immunoprecipitation (IP)
(ChIP analysis of estrogen target genes. (A) Soluble chromatin was prepared from MCF7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed using antibody MBS643679. DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter. Adapted from Fig 6 in: Carling et al., 2004. Mol Cell Bio. 24 (16): 7032-7042.)
Immunoprecipitation (IP)
(ChIP analysis of estrogen target genes. (B) Time course analysis of RIZ1 binding to the pS2 gene promoter. MCF7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis. Immunoprecipitation was performed using antibody MBS643679 and control immunoglobulin G as indicated. Adapted from Fig 6 in: Carling et al., 2004. Mol Cell Bio. 24 (16): 7032-7042.)
Immunoprecipitation (IP)
(ChIP analysis of estrogen target genes. (B) Time course analysis of RIZ1 binding to the pS2 gene promoter. MCF7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis. Immunoprecipitation was performed using antibody MBS643679 and control immunoglobulin G as indicated. Adapted from Fig 6 in: Carling et al., 2004. Mol Cell Bio. 24 (16): 7032-7042.)
Immunoprecipitation (IP)
(ChIP analysis of estrogen target genes. (C) ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts using antibody MBS643679. Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the cdc25c promoter region. Adapted from Fig 6 in: Carling et al., 2004. Mol Cell Bio. 24 (16): 7032-7042.)
Immunoprecipitation (IP)
(ChIP analysis of estrogen target genes. (C) ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts using antibody MBS643679. Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the cdc25c promoter region. Adapted from Fig 6 in: Carling et al., 2004. Mol Cell Bio. 24 (16): 7032-7042.)
Related Product Information for anti-Prdm2 antibody
This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene. Although the functions of this protein have not been fully characterized, it may (1) play a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma, (2) act as a transcriptional activator of the heme-oxygenase-1 gene, and (3) be a specific effector of estrogen action. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-Prdm2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Prdm2 protein
NCBI Official Synonym Full Names
PR domain containing 2, with ZNF domain
NCBI Official Symbol
Prdm2
NCBI Official Synonym Symbols
Riz; KMT8; Riz1; Gm1033; Gm19732; Znfpr1c1; 4833427P12Rik; E330024L24Rik
NCBI Protein Information
retinoblastoma protein-binding zinc finger protein
Protein Family
Research Articles on Prdm2
Similar Products
Product Notes
The Prdm2 (Catalog #AAA643679) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PRDM2 (RIZ1) (PRDM2, KMT8, RIZ, PR domain zinc finger protein 2, GATA-3-binding protein G3B, Lysine N-methyltransferase 8, MTB-ZF, MTE-binding protein, PR domain-containing protein 2, Retinoblastoma protein-interacting zinc finger protein, Zinc finger pro reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRDM2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot, Immunoprecipitation, ELISA. Dilution: ELISA: 1:1,000 Western Blot: 1:100~500 Immuno precipitation. Researchers should empirically determine the suitability of the Prdm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRDM2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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