Mouse Postsynaptic Density Protein 95 Monoclonal Antibody | anti-DLG4 antibody

Postsynaptic Density Protein 95 (PSD-95, PSD95, Discs Large Homolog 4, DLG4, Post-synaptic Density Protein 95, Synapse-associated Protein 90, SAP90, SAP-90)

Cat. Num. AAA603923

• Gene Names: DLG4; PSD95; SAP90; SAP-90
• Reactivity: Bovine, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Postsynaptic Density Protein 95; Monoclonal Antibody; Postsynaptic Density Protein 95 (PSD-95; PSD95; Discs Large Homolog 4; DLG4; Post-synaptic Density Protein 95; Synapse-associated Protein 90; SAP90; SAP-90); Anti -Postsynaptic Density Protein 95 (PSD-95; anti-DLG4 antibody

• Host: Mouse
• Reactivity: Bovine, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 4E181
• Specificity: Detects a ~100kD protein, corresponding to the apparent molecular mass of PSD-95 on SDS-PAGE immunoblots, in samples from mouse, rat and bovine. A molecular mass band of ~75kD is also present in rat brain and mouse brain tissue extracts.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH7.2, 0.1mM PMSF, 50% glycerol.

• Applicable Applications for anti-DLG4 antibody: Western Blot (WB)
• Application Notes: Western Blot (ECL): 5 ug/ml, Immunoctyochemistry (3,4): 12.5 ug/ml 1:500, Optimal dilutions to be determined by researcher.
• Immunogen: Purified recombinant rat PSD-95
• Positive Control: Mouse Brain Tissue Extract; Rat Brain Tissue Extract
• Preparation and Storage: -20 degree C

Related Product Information for anti-DLG4 antibody

Postsynaptic density protein 95kD (PSD-95), also known as Synapse associated protein 90kD (SAP90), is a brain specific protein that is highly similar to the Drosophila dlg tumor suppressor protein (1). PSD-95 is a member of membrane associated proteins that are localized beneath the postsynaptic membrane of synapses in the CNS. PSD-95 contains a carboxyl-terminal guanylate kinase domain, an upstream SH3 domain, and three amino-terminal PDZ domains. PSD-95 interacts with NMDA receptor and Shaker-type K+ channel and contributes to their clustering and localization at the synaptic spines in hippocampal neurons and the pinceau terminal of cerebellar basket cells, respectively (2). The yeast two hybrid method revealed that the second PDZ domain in PSD-95 binded tightly to the carboxyl terminal (t)-S/TXV sequence of the NR2B subunit of NMDA receptor and of Shaker-type K+ channel. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interaction between PDZ domains present on both proteins.

Product Categories/Family for anti-DLG4 antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 3318653
• NCBI GeneID: 1742
• UniProt Accession #: P78352; Q92941; Q9UKK8; B7Z1S1; G5E939
• Molecular Weight: 80,495 Da
• NCBI Official Full Name: post-synaptic density protein 95
• NCBI Official Synonym Full Names: discs, large homolog 4 (Drosophila)
• NCBI Official Symbol: DLG4
• NCBI Official Synonym Symbols: PSD95; SAP90; SAP-90
• NCBI Protein Information: disks large homolog 4; discs large homolog 4; Tax interaction protein 15; synapse-associated protein 90; postsynaptic density protein 95; post-synaptic density protein 95
• UniProt Protein Name: Disks large homolog 4
• Protein Family: Disks large
• UniProt Gene Name: DLG4
• UniProt Synonym Gene Names: PSD95; PSD-95; SAP-90; SAP90
• UniProt Entry Name: DLG4_HUMAN

NCBI Description

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PSD-95: a membrane associated guanylate kinase (MAGUK) scaffolding protein located in neural postsynaptic densities. Associates with NMDA receptor NR2 subunits via glutamate serine (aspartate/glutamate) valine motifs in the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Its overexpression or depletion changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. Binds tissue-type plasminogen activator (tPA), mediating neural NMDA gating via a complex of LRP1, PSD-95 and NMDAR. Interacts through its first two PDZ domains with NMDAR2A, NMDAR2B, NMDAR2C, NMDAR2D, ACCN3, certain splice forms of NMDAR1, KV4.2, CXADR and synGAP. Interacts through its first two PDZ domains with Kv1.1, Kv1.2, Kv1.3, Kv1.4 and HER4. Interacts through its first PDZ domain with GluR6 and Kv1.4. Interacts through its second PDZ domain with the PDZ domain of nNOS or the C-terminus of NOS1AP. May interact with 5-HT(2A). Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. Interacts through its guanylate kinase-like domain with SAPAP1, SAPAP2, SAPAP3, SAPAP4, MAP1A, and BEGAIN. Interacts through its guanylate kinase-like domain with KIF13B. Two alternatively spliced human isoforms have been reported. Palmitoylation of isoform 1 is required for targeting to postsynaptic density.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: dendrite cytoplasm; cortical cytoskeleton; voltage-gated potassium channel complex; basolateral plasma membrane; endoplasmic reticulum; postsynaptic density; dendritic spine; excitatory synapse; postsynaptic membrane; synaptic vesicle; extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; ionotropic glutamate receptor complex; synapse; cell junction

Molecular Function: protein C-terminus binding; P2Y1 nucleotide receptor binding; ionotropic glutamate receptor binding; guanylate kinase activity; protein binding; beta-1 adrenergic receptor binding; protein complex binding; acetylcholine receptor binding; D1 dopamine receptor binding; protein phosphatase binding; PDZ domain binding

Biological Process: regulation of long-term neuronal synaptic plasticity; nervous system development; axon guidance; synaptic vesicle maturation; learning; social behavior; signal transduction; positive regulation of synaptic transmission; establishment and/or maintenance of epithelial cell polarity; synaptic transmission; establishment of protein localization; elevation of cytosolic calcium ion concentration; protein complex assembly; neuromuscular process controlling balance; negative regulation of receptor internalization; nucleotide phosphorylation