Immunohistochemistry (IHC)
(Anti-PNPO antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Mouse anti-Human PNPO Monoclonal Antibody | anti-PNPO antibody
Mouse Monoclonal [clone AT2C7] (IgG1,k) to Human PNPO
Immunohistochemistry (IHC)
(Anti-PNPO antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Immunohistochemistry (IHC)
(Anti-PNPO antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Western Blot (WB)
(Western Blot: The extracts of HepG2 (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNPO (1:500-1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(Western Blot: The extracts of HepG2 (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNPO (1:500-1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
NCBI and Uniprot Product Information
NCBI Description
The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq, Oct 2008]
Uniprot Description
PNPO: Catalyzes the oxidation of either pyridoxine 5'- phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP). Defects in PNPO are the cause of pyridoxine-5'-phosphate oxidase deficiency (PNPO deficiency); also known as PNPO-related neonatal epileptic encephalopathy. The main feature of neonatal epileptic encephalopathy is the onset within hours of birth of a severe seizure disorder that does not respond to anticonvulsant drugs and can be fatal. Seizures can cease with the administration of PLP, being resistant to treatment with pyridoxine. Belongs to the pyridoxamine 5'-phosphate oxidase family.
Protein type: EC 1.4.3.5; Cofactor and Vitamin Metabolism - vitamin B6; Oxidoreductase
Chromosomal Location of Human Ortholog: 17q21.32
Cellular Component: nucleoplasm; cytoplasm; cytosol
Molecular Function: FMN binding; pyridoxamine-phosphate oxidase activity
Biological Process: vitamin metabolic process; vitamin B6 metabolic process; pyridoxine biosynthetic process; water-soluble vitamin metabolic process; pyridoxal phosphate biosynthetic process
Disease: Pyridoxamine 5-prime-phosphate Oxidase Deficiency
Research Articles on PNPO
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Product Notes
The PNPO pnpo (Catalog #AAA249155) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone AT2C7] (IgG1,k) to Human PNPO reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PNPO can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA, IHC-P (10 ug/ml), WB (1:500 - 1:5000). Researchers should empirically determine the suitability of the PNPO pnpo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PNPO, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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