Testing Data
Mouse PLCg1 (N-terminal Region) Monoclonal Antibody | anti-PLCG1 antibody
Anti-PLC-gamma1 (N-terminal region)
Immunocytochemistry: 1:100
Immunoprecipitation: 1-5ml
Western Blot: 1:1000
Testing Data
Testing Data
• Chen, P. et al. (1996) J Cell. Biol. 134:689.
• Serrano, C.J. et al. (2005) J. Immunol. 174:6233.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Uniprot Description
Function: Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Ref.17
Catalytic activity: 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
Cofactor: Calcium.
Subunit structure: Interacts with AGAP2 via its SH3 domain
By similarity. Interacts with phosphorylated LAT upon TCR activation. Interacts with the Pro-rich domain of TNK1 via its SH3 domain. Associates with BLNK, VAV1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB in activated T-cells; which inhibits phosphorylation. Interacts with SHB. Interacts via its SH3 domain with the Arg/Gly-rich-flanked Pro-rich domains of KHDRBS1/SAM68. This interaction is selectively regulated by arginine methylation of KHDRBS1/SAM68. Interacts with INPP5D/SHIP1, THEMIS and CLNK
By similarity. Interacts with RALGPS1. Interacts (via SH3 domain) with HEV ORF3 protein. Interacts (via the SH2 domains) with VIL1 (phosphorylated at C-terminus tyrosine phosphorylation sites). Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.13 Ref.17
Subcellular location: Cell projection › lamellipodium. Cell projection › ruffle. Note: Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. Ref.17
Domain: The SH3 domain mediates interaction with CLNK
By similarity. The SH3 domain also mediates interaction with RALGPS1. Ref.26
Post-translational modification: The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ. Ref.11 Ref.12 Ref.14 Ref.15 Ref.16 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24Ubiquitinated by CBLB in activated T-cells
Sequence similarities: Contains 1 C2 domain.Contains 1 EF-hand domain.Contains 2 PH domains.Contains 1 PI-PLC X-box domain.Contains 1 PI-PLC Y-box domain.Contains 2 SH2 domains.Contains 1 SH3 domain.
Research Articles on PLCG1
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Product Notes
The PLCG1 plcg1 (Catalog #AAA470026) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-PLC-gamma1 (N-terminal region) reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PLCg1 (N-terminal Region) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunocytochemistry, Immunoprecipitation. ELISA: 1:2000 Immunocytochemistry: 1:100 Immunoprecipitation: 1-5ml Western Blot: 1:1000. Researchers should empirically determine the suitability of the PLCG1 plcg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLCg1 (N-terminal Region), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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