Mouse Plakoglobin Monoclonal Antibody | anti-JUP antibody

Plakoglobin, Human, mAb 15F11

Cat. Num. AAA584165

• Gene Names: JUP; DP3; PDGB; PKGB; CTNNG; DPIII; ARVD12
• Reactivity: Reactivity: Human. Cross reactivity: Mouse (Weak), Rat
• Applications: Immunofluorescence, Immunoprecipitation, Western Blot
• Synonyms: Plakoglobin; Monoclonal Antibody; Human; mAb 15F11; anti-JUP antibody

• Host: Mouse
• Reactivity: Reactivity: Human. Cross reactivity: Mouse (Weak), Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 744

• Applicable Applications for anti-JUP antibody: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Application Use: For Western blotting and immunofluorescence dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-JUP antibody

Product Description: Plakoglobin, also known as gamma-catenin belongs together with alpha- and beta catenin to the catenin family. Catenins mediates cell-cell adhesion by interaction with cadherins. Plakoglobin is found in desmosomes and adherens junctions. Plakoglobin is highly homologous to beta-catenin although its function differs from that of beta-catenin. Whereas beta-catenin has been found in potentiating hyperproliferation and tumor formation, plakoglobin can suppress tumorigenicity. Overexpression of plakoglobin has been shown to suppress cell proliferation and cell tumorigenicity in animals. Furthermore reduced plakoglobin expression has been found in tumor tissues and metastatic lesions of renal cells, esophageal carcinomas and in skin carcinomas. The monoclonal antibody 15F11 cross reacts with rat and weakly with mouse.

References

1. Sacco, P et al; Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin. J Biol Chem 1995, 34: 20201 2. Wahl, J et al; Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains. J Cell Sci 1996, 109: 1143
3. Lewis, J et al; Cross-talk between adherens junctions and desmosomes depends on plakoglobin. J Cell Biol 1997, 136: 919
4. Amitay, R et al; Reduced expression of plakoglobin correlates with adverse outcome in patients with neuroblastoma. Am J Pathol 2001, 159: 43

NCBI and Uniprot Product Information

• NCBI GI #: 762885
• NCBI GeneID: 3728
• Molecular Weight: 81,745 Da
• NCBI Official Full Name: Plakoglobin
• NCBI Official Synonym Full Names: junction plakoglobin
• NCBI Official Symbol: JUP
• NCBI Official Synonym Symbols: DP3; PDGB; PKGB; CTNNG; DPIII; ARVD12
• NCBI Protein Information: junction plakoglobin; desmoplakin-3; desmoplakin III; catenin (cadherin-associated protein), gamma 80kDa
• UniProt Protein Name: Junction plakoglobin
• Protein Family: Junction plakoglobin
• UniProt Gene Name: JUP
• UniProt Synonym Gene Names: CTNNG; DP3
• UniProt Entry Name: PLAK_HUMAN

NCBI Description

This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described. [provided by RefSeq, Jul 2008]

Uniprot Description

CTNNG: Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. Homodimer. Component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1 and gamma- catenin/JUP, and possibly alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Interacts with MUC1. Interacts with CAV1. Interacts with PTPRJ. Interacts with DSC2. Belongs to the beta-catenin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: desmosome; internal side of plasma membrane; focal adhesion; apicolateral plasma membrane; intermediate filament; zonula adherens; fascia adherens; catenin complex; intercellular junction; Z disc; cytosol; actin cytoskeleton; cell-cell adherens junction; cytoskeleton; hemidesmosome; cytoplasm; plasma membrane; nucleus; lateral plasma membrane

Molecular Function: signal transducer activity; protein binding; protein homodimerization activity; structural constituent of cell wall; cadherin binding; transcription coactivator activity; structural molecule activity; protein kinase binding; protein phosphatase binding; nuclear hormone receptor binding; alpha-catenin binding

Biological Process: skin development; intercellular junction assembly and maintenance; cell migration; cell-cell adhesion; positive regulation of protein import into nucleus; protein heterooligomerization; cytoskeletal anchoring; regulation of cell fate specification; detection of mechanical stimulus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; vascular endothelial growth factor receptor signaling pathway; regulation of cell proliferation

Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 12; Naxos Disease