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FCM (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells with PIST antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human, Mouse PIST Monoclonal Antibody | anti-PIST antibody
PIST Antibody
Gene Names
GOPC; CAL; FIG; PIST; GOPC1; dJ94G16.2
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
PIST; Monoclonal Antibody; PIST Antibody; CAL antibody; CFTR associated ligand antibody; CFTR-associated ligand antibody; dJ94G16.2 antibody; dJ94G16.2 PIST antibody; FIG antibody; Fused in glioblastoma antibody; Golgi associated PDZ and coiled coil motif containing antibody; Golgi associated PDZ and coiled coil motif containing protein antibody; Golgi-associated PDZ and coiled-coil motif-containing protein antibody; GOPC 1 antibody; GOPC antibody; GOPC_HUMAN antibody; GOPC1 antibody; OTTHUMP00000040403 antibody; PDZ protein interacting specifically with TC 10 antibody; PDZ protein interacting specifically with TC10 antibody; PDZ/coiled coil domain binding partner for the rho family GTPase TC 10 antibody; PDZ/coiled coil domain binding partner for the rho family GTPase TC10 antibody; PIST antibody; Protein interacting specifically with Tc 10 antibody; Protein interacting specifically with Tc10 antibody; anti-PIST antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
JG79-35
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PIST antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
IP: 1:10-1:50
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
IP: 1:10-1:50
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein within human PIST aa 1-100.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells with PIST antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells with PIST antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining PIST in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PIST in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PIST in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PIST in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PIST in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PIST in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PIST antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PIST antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PIST antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PIST antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PIST antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PIST antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PIST antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PIST antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of PIST on SiHa cell using anti-PIST antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of PIST on SiHa cell using anti-PIST antibody at 1/1, 000 dilution.)
Related Product Information for anti-PIST antibody
PIST (PDZ protein interacting specifically with TC10), also known as GOPC (golgi associated PDZ and coiled-coil motif containing), CAL or FIG, is a 462 amino acid protein that localizes to the cytoplasm, as well as to the membrane of the golgi apparatus and to the cell junction. Expressed ubiquitously and containing one PDZ (DHR) domain, PIST functions as a homooligomer that interacts with a variety of proteins and plays a role in intracellular protein trafficking and degradation. Additionally, PIST is thought to regulate ionic currents via membrane channel modification and may also play a role in autophagy. Chromosomal aberrations in the gene encoding PIST are found in glioblastoma multiform (GBM), a common and aggressive form of brain tumor, suggesting a role for mutated PIST in carcinogenesis. Three isoforms of PIST exist due to alternative splicing events.
Product Categories/Family for anti-PIST antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
462
NCBI Official Full Name
Golgi-associated PDZ and coiled-coil motif-containing protein
NCBI Official Synonym Full Names
golgi-associated PDZ and coiled-coil motif containing
NCBI Official Symbol
GOPC
NCBI Official Synonym Symbols
CAL; FIG; PIST; GOPC1; dJ94G16.2
NCBI Protein Information
Golgi-associated PDZ and coiled-coil motif-containing protein; dJ94G16.2 PIST; fused in glioblastoma; CFTR-associated ligand; PDZ protein interacting specifically with TC10; Golgi associated PDZ and coiled-coil motif containing protein; golgi-associated P
UniProt Protein Name
Golgi-associated PDZ and coiled-coil motif-containing protein
UniProt Gene Name
GOPC
UniProt Synonym Gene Names
CAL; FIG; PIST
UniProt Entry Name
GOPC_HUMAN
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Product Notes
The PIST gopc (Catalog #AAA30504) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIST Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PIST can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 IP: 1:10-1:50 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the PIST gopc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIST, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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