Western Blot (WB)
(Western blot image of human A431 cells. The blots were probed with mouse monoclonal anti-PI3 Kinase, p85 (C-terminal region) at 1:1000 (lane 1), 1:2000 (lane 2), and 1:4000 (lanes 3).)
Mouse PI3 Kinase, p85 Monoclonal Antibody | anti-PI3 antibody
PI3 Kinase, p85 (C-terminal region) Antibody
ICC: 1:100
WB: 1:1000
Western Blot (WB)
(Western blot image of human A431 cells. The blots were probed with mouse monoclonal anti-PI3 Kinase, p85 (C-terminal region) at 1:1000 (lane 1), 1:2000 (lane 2), and 1:4000 (lanes 3).)
Western Blot (WB)
(Western blot image of human A431 cells. The blots were probed with mouse monoclonal anti-PI3 Kinase, p85 (C-terminal region) at 1:1000 (lane 1), 1:2000 (lane 2), and 1:4000 (lanes 3).)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of PI3 Kinase p85 in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal PI3 Kinase p85 (C-terminal region) antibody (PM2531), then the antibody was detected using appropriate secondary antibody conjugated to DyLight 594.)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of PI3 Kinase p85 in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal PI3 Kinase p85 (C-terminal region) antibody (PM2531), then the antibody was detected using appropriate secondary antibody conjugated to DyLight 594.)
Stoyanov, B. et al. (1995) Science 269:690.
NCBI and Uniprot Product Information
NCBI Description
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
Uniprot Description
PIK3R1: regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids. May play an additional role in the regulation of the actin cytoskeleton. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Its SH2 domains interacts with the YTHM motif of phosphorylated INSR in vitro. Defects in PIK3R1 are a cause of severe insulin resistance. Four alternatively spliced isoforms have been described.
Protein type: Enzyme, regulatory subunit; Kinase, lipid; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 5q13.1
Cellular Component: membrane; cytoplasm; plasma membrane; intercellular junction; phosphoinositide 3-kinase complex; nucleus; cytosol
Molecular Function: insulin binding; insulin-like growth factor receptor binding; protein binding; ErbB-3 class receptor binding; neurotrophin TRKA receptor binding; insulin receptor substrate binding; phosphoinositide 3-kinase binding; phosphoinositide 3-kinase regulator activity; transmembrane receptor protein tyrosine kinase adaptor protein activity; transcription factor binding; protein phosphatase binding; insulin receptor binding
Biological Process: negative regulation of osteoclast differentiation; phosphoinositide 3-kinase cascade; viral reproduction; nerve growth factor receptor signaling pathway; protein amino acid phosphorylation; T cell receptor signaling pathway; positive regulation of RNA splicing; regulation of phosphoinositide 3-kinase activity; NFAT protein import into nucleus; positive regulation of glucose import; phosphoinositide phosphorylation; phospholipid metabolic process; positive regulation of transcription factor import into nucleus; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; protein stabilization; negative regulation of cell-matrix adhesion; positive regulation of tumor necrosis factor production; DNA damage response, signal transduction resulting in induction of apoptosis; insulin-like growth factor receptor signaling pathway; induction of apoptosis via death domain receptors; cellular response to insulin stimulus; B cell differentiation; T cell costimulation; insulin receptor signaling pathway; phosphatidylinositol biosynthetic process; innate immune response; cell glucose homeostasis; positive regulation of transcription from RNA polymerase II promoter; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration; positive regulation of cell migration; negative regulation of apoptosis
Disease: Agammaglobulinemia 7, Autosomal Recessive; Short Syndrome; Immunodeficiency 36
Research Articles on PI3
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Product Notes
The PI3 pik3r1 (Catalog #AAA474517) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PI3 Kinase, p85 (C-terminal region) Antibody reacts with Human, Rat, Mouse, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's PI3 Kinase, p85 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). ELISA: 1:2000 ICC: 1:100 WB: 1:1000. Researchers should empirically determine the suitability of the PI3 pik3r1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI3 Kinase, p85, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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