Testing Data
Mouse anti-Human Phosphotyrosine Ascites Monoclonal Antibody
Anti-Phosphotyrosine Ascites Antibody
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Ascites
Synonyms
Phosphotyrosine Ascites; Monoclonal Antibody; Anti-Phosphotyrosine Ascites Antibody; Phosphotyrosine; anti-Phosphotyrosine Ascites antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Specificity
This product is clarified ascites produced in balb/c mice using clone 13F9. Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphoserine or phosphothreonine.
Purity/Purification
Ascites
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Phosphotyrosine Ascites antibody
ELISA (EIA), Western Blot (WB)
Application Notes
This monoclonal antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphothreonine. The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation. Phosphorylation of tyrosine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.
Immunogen
This monoclonal antibody was produced after repeated immunizations of balb/c mice with phosphotyrosine conjugated KLH.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Testing Data
Testing Data
Similar Products
Product Notes
The Phosphotyrosine Ascites (Catalog #AAA4157027) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phosphotyrosine Ascites Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphotyrosine Ascites can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). This monoclonal antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphothreonine. The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation. Phosphorylation of tyrosine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation. Researchers should empirically determine the suitability of the Phosphotyrosine Ascites for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphotyrosine Ascites, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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