Mouse Phosphotyrosine Monoclonal Antibody
Phosphotyrosine, mixed clones (MaxLight 650)
Applications
Immunoprecipitation, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
Phosphotyrosine; Monoclonal Antibody; mixed clones (MaxLight 650); anti-Phosphotyrosine antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3G244
Specificity
PY-Plus reacts specifically with tyrosine-phosphorylated proteins. It shows no crossreactivity to phosphoserine or phosphothreonine. On Western blots, specific inhibition of each individual antibody and the cocktail reactivity can be achieved by pre-incubation of the antibody with 20 mM phosphotyrosine, whereas pre-incubation with 20 mM phosphoserine or 20 mM phosphothreonine is ineffective. Species crossreactivity: It is well established that no one monoclonal antibody can strongly react with all tyrosine-phosphorylated proteins. PY-Plus contains 2 different monoclonal antibodies that are designed to strongly react with the broadest range of tyrosinephosphorylated proteins possible. It will react with tyrosine-phosphorylated proteins in both the native and denatured states. Positive reactivity is independent of the species source and has been observed in the following samples: mouse brain, NIH 3T3 cells (+/-TPA), K562 cells, EGF-stimulated A431 cells and MCF-7 cells.
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight650.
Applicable Applications for anti-Phosphotyrosine antibody
Immunoprecipitation (IP), Western Blot (WB)
Immunogen
Tyrosine-phosphorylated proteins
Conjugate
MaxLight650
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Product Categories/Family for anti-Phosphotyrosine antibody
References
1. Frackelton AR, et al. Mol Cell Biol 3:1343-1352, 1983. 2. Daniel TO, et al. PNAS 82:2684-2687, 1985. 3. Hunter T, et al. Cell 50:823-829, 1987. 4. Glenny JR, et al. J Immunol Meth 109:277-285, 1988. 5. Sengupta A, et al. PNAS 85:8062-8066, 1988. 6. Hunter T, et al. Meth Enzymol 200:3-37, 1991. 7. Sun H, et al. Trends Biochem Sci 19:480-485, 1994. 8. Bourguignon LY, et al. J Biol Chem 272(44):27913-27918, 1997. 9. Hua CI, et al. J Biol Chem 273(43):283332-28340, 1998. 10. Conus NM, et al. J Biol Chem 273(8):4776-4782, 1998. 11. Tava S, et al. J Cell Biol 155(5):809-819, 2001. 12. Bourguignon L, et al. J Biol Chen 276(10):7327-7336, 2001. 13. Gilbertson DG, et al. J Biol Chem 276(29):27406-27414, 2001. 14. Edens HA, et al. J Immunol 169:476-486, 2002. 15. Conus NM, et al. J Biol Chem 277(41):38021-38028, 2002. 16. Del Valle, et al. J Biol Chem 278(19):17430-17437, 2003.
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Product Notes
The Phosphotyrosine (Catalog #AAA6259744) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phosphotyrosine can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the Phosphotyrosine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphotyrosine, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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