Western Blot (WB)
Mouse anti-Human, Yeast Phospho-RNA polymerase II CTD repeat YSPTSPS Monoclonal Antibody | anti-POLR2A antibody
Phospho-RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody (CTD 8A7
Reactivity
Human, Yeast
Applications
Immunofluorescence, Western Blot
Synonyms
Phospho-RNA polymerase II CTD repeat YSPTSPS; Monoclonal Antibody; Phospho-RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody (CTD 8A7; RNA Polymerase II; anti-POLR2A antibody
Host
Mouse
Reactivity
Human, Yeast
Clonality
Monoclonal
Isotype
Mouse IgM
Clone Number
CTD8A7
Specificity
Monoclonal antibody to detect RNA polII (phospho Ser5). Monoclonal antibody 8A7 was obtained using synthetic phosphopeptides and specifically recognises the Ser-5 phosphorylated form of RNAP II
Form/Format
Colorless liquid.
Applicable Applications for anti-POLR2A antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
WB: 1:1000
Immunogen
10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
Positive Control
WB: HeLa cell lysate
Preparation and Storage
Store at -20 degree C
The antibody solution should be gently mixed before use
Shelf life: ~12 months
The antibody solution should be gently mixed before use
Shelf life: ~12 months
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-POLR2A antibody
RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. Mutations that directly affect transcription by RNA polymerases rank among the most central mediators of malignant transformation.
Product Categories/Family for anti-POLR2A antibody
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Product Notes
The POLR2A (Catalog #AAA847163) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Phospho-RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody (CTD 8A7 reacts with Human, Yeast and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-RNA polymerase II CTD repeat YSPTSPS can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the POLR2A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-RNA polymerase II CTD repeat YSPTSPS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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