Mouse anti-Human PGR/PR Monoclonal Antibody | anti-PGR antibody

PGR/PR (PGR, NR3C3, Progesterone receptor, Nuclear receptor subfamily 3 group C member 3)

Cat. Num. AAA6008779

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: PGR/PR; Monoclonal Antibody; PGR/PR (PGR; NR3C3; Progesterone receptor; Nuclear receptor subfamily 3 group C member 3); Anti -PGR/PR (PGR; anti-PGR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgM,k
• Clone Number: 312CT8.2.1.6
• Specificity: Human
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

• Applicable Applications for anti-PGR antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA.; Dilution: ELISA: 1:1000; Western Blot: 1:100
• Immunogen: PGR Mab is generated from mouse immunized with PGR recombinant protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(PGR/PR Antibody western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the PGR/PR antibody detected the PGR/PR protein (arrow).)

Related Product Information for anti-PGR antibody

This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. The location of transcription initiation for isoform A has not been clearly determined.

Product Categories/Family for anti-PGR antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 126722780
• NCBI GeneID: 100009094
• NCBI Accession #: NP_001075736.1
• NCBI GenBank Nucleotide #: NM_001082267.1
• UniProt Accession #: P06186
• Molecular Weight: 98,667 Da
• NCBI Official Full Name: progesterone receptor
• NCBI Official Symbol: PGR
• NCBI Protein Information: progesterone receptor; PR; nuclear receptor subfamily 3 group C member 3
• UniProt Protein Name: Progesterone receptor
• Protein Family: Protein
• UniProt Gene Name: PGR
• UniProt Synonym Gene Names: NR3C3; PR
• UniProt Entry Name: PRGR_RABIT

Uniprot Description

Function: The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor is involved in activation of c-SRC/MAPK signaling on hormone stimulation

By similarity.

Subunit structure: Interacts with SMARD1 and UNC45A. Interacts with CUEDC2; the interaction promotes ubiquitination, decreases sumoylation, and repesses transcriptional activity. Interacts with PIAS3; the interaction promotes sumoylation of PR in a hormone-dependent manner, inhibits DNA-binding, and alters nuclear export. Interacts with SP1; the interaction requires ligand-induced phosphorylation on Ser-344. Interacts with PRMT2

By similarity.

Subcellular location: Nucleus. Cytoplasm. Note: Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G1 and G2/M phases

By similarity.

Domain: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

Post-translational modification: Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-293 is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-387. Phosphorylation on Ser-102 and Ser-344 also requires induction by hormone. Basal phosphorylation on Ser-82, Ser-191 and Ser-399 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-399 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-293, but not at Ser-191, is impaired during the G2/M phase of the cell cycle. Phosphorylation on Ser-344 by ERK1/2 MAPK is required for interaction with SP1

By similarity.Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-387, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-293

By similarity.Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-293

By similarity.Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation

By similarity.

Sequence similarities: Belongs to the nuclear hormone receptor family. NR3 subfamily.Contains 1 nuclear receptor DNA-binding domain.