Mouse anti-Human PGM1 Monoclonal Antibody | anti-PGM1 antibody

PGM1 antibody

Cat. Num. AAA200405

• Gene Names: PGM1; CDG1T; GSD14
• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
• Purity: By protein-A affinity chromatography
• Synonyms: PGM1; Monoclonal Antibody; PGM1 antibody; Phosphoglucomutase-1 isoform 1; CDG1T; GSD14; anti-PGM1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: AT84G2
• Purity/Purification: By protein-A affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 562

• Applicable Applications for anti-PGM1 antibody: ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Antigen Species: Human
• Immunogen: Recombinant human PGM1 (1-562aa) purified from E. coli
• Clone Note: Anti-human PGM1 mAb, clone AT84G2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PGM1 protein.
• Preparation and Storage: Can be stored at 4 degree C.; For long term storage, aliquot and store at -20 degree C.; Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : HepG2 cell lysate Lane 3. : NIH/3T3 cell lysate Lane 4. : Jurkat cell lysate Lane 5. : HeLa cell lysate Lane 6. : U87MG cell lysate )

Immunofluorescence (IF)

(ICC/IF analysis of PGM1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGM1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

Related Product Information for anti-PGM1 antibody

PGM1 is an evolutionarily conserved enzyme that regulates one of the most important metabolic carbohydrate trafficking points in prokaryotic and eukaryotic organisms, catalyzing the bi-directional interconversion of glucose 1-phosphate (G-1-P) and glucose 6-phosphate (G-6-P). In one direction, G-1-P produced from sucrose catabolism is converted to G-6-P, the first intermediate in glycolysis. In the other direction, conversion of G-6-P to G-1-P generates a substrate for synthesis of UDP-glucose, which is required for synthesis of a variety of cellular constituents, including cell wall polymers and glycoproteins. PGM1 has been used extensively as a genetic marker for isozyme polymorphism among humans. PGM is known to be post-translationally modified by cytoplasmic glycosylation that does not seem to regulate its enzymatic activity but rather is implicated in the localization of the protein. Glucose 1,6 bisphosphate (Glc-1, 6-P2), a powerful regulator of carbohydrate metabolism, has been demonstrated to be a potent activator of PGM. PGM1 is also modified by phosphorylation on Ser108 as part of its catalytic mechanism. This is shown to be performed by Pak1, a previously identified signaling kinase.

Product Categories/Family for anti-PGM1 antibody

Signal Transduction; Metabolism

References

Boros LG., et al. (2002) Pancreas. 24 (1): 26-33.; Dey NB., et al. (1994) The Journal of Biological Chemistry. 269 (43): 27143-8.; Gururaj A., et al. (2004) Oncogene. 23 (49): 8118-27.

NCBI and Uniprot Product Information

• NCBI GI #: 21361621
• NCBI GeneID: 5236
• NCBI Accession #: NP_002624
• NCBI GenBank Nucleotide #: NM_002633.2
• UniProt Accession #: P36871; Q16105; Q16106; Q5BKZ9; Q6NW22; Q86U74; Q96J40; Q9NTY4; B2R5N9; B4DPV0
• Molecular Weight: 40,283 Da
• NCBI Official Full Name: phosphoglucomutase-1 isoform 1
• NCBI Official Synonym Full Names: phosphoglucomutase 1
• NCBI Official Symbol: PGM1
• NCBI Official Synonym Symbols: CDG1T; GSD14
• NCBI Protein Information: phosphoglucomutase-1
• UniProt Protein Name: Phosphoglucomutase-1
• Protein Family: Phosphoglucomutase
• UniProt Gene Name: PGM1
• UniProt Synonym Gene Names: PGM 1

NCBI Description

The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010]

Uniprot Description

This enzyme participates in both the breakdown and synthesis of glucose.