Mouse anti-Human PGM1 Monoclonal Antibody | anti-PGM1 antibody

PGM1 (Phosphoglucomutase-1, Glucose Phosphomutase 1) (Biotin)

Cat. Num. AAA6143506

• Gene Names: PGM1; CDG1T; GSD14
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PGM1; Monoclonal Antibody; PGM1 (Phosphoglucomutase-1; Glucose Phosphomutase 1) (Biotin); anti-PGM1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3B8-H4
• Specificity: Recognizes human PGM1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PGM1 antibody: ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IF: 20ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-563 from human PGM1 (AAH19920) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MVKIVTVKTQAYQDQKPGTSGLRKRVKVFQSSANYAENFIQSIISTVEPAQRQEATLVVGGDGRFYMKEAIQLIARIAAANGIGRLVIGQNGILSTPAVSCIIRKIKAIGGIILTASHNPGGPNGDFGIKFNISNGGPAPEAITDKIFQISKTIEEYAVCPDLKVDLGVLGKQQFDLENKFKPFTVEIVDSVEAYATMLRSIFDFSALKELLSGPNRLKIRIDAMHGVVGPYVKKILCEELGAPANSAVNCVPLEDFGGHHPDPNLTYAADLVETMKSGEHDFGAAFDGDGDRNMILGKHGFFVNPSDSVAVIAANIFSIPYFQQTGVRGFARSMPTSGALDRVASATKIALYETPTGWKFFGNLMDASKLSLCGEESFGTGSDHIREKDGLWAVLAWLSILATRKQSVEDILKDHWQKYGRNFFTRYDYEEVEAEGANKMMKDLEALMFDRSFVGKQFSANDKVYTVEKADNFEYSDPVDGSISRNQGLRLIFTDGSRIVFRLSGTGSAGATIRLYIDSYEKDVAKINQDPQVMLAPLISIALKVSQLQERTGRTAPTVIT
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (87.56kD).)

Western Blot (WB)

(PGM1 monoclonal antibody Western Blot analysis of PGM1 expression in Jurkat.)

Western Blot (WB)

(Western Blot analysis of PGM1 expression in transfected 293T cell line by PGM1 monoclonal antibody. Lane 1: PGM1 transfected lysate (61.4kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to PGM1 on HeLa cell. [antibody concentration 20ug/ml])

Immunoprecipitation (IP)

(Immunoprecipitation of PGM1 transfected lysate using PGM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM1 rabbit polyclonal antibody)

Testing Data

(Detection limit for recombinant GST tagged PGM1 is 0.3ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Product Categories/Family for anti-PGM1 antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 18043711
• NCBI GeneID: 5236
• Molecular Weight: 40,283 Da
• NCBI Official Full Name: Homo sapiens phosphoglucomutase 1, mRNA
• NCBI Official Synonym Full Names: phosphoglucomutase 1
• NCBI Official Symbol: PGM1
• NCBI Official Synonym Symbols: CDG1T; GSD14
• NCBI Protein Information: phosphoglucomutase-1; PGM 1; glucose phosphomutase 1
• Protein Family: Phosphoglucomutase

NCBI Description

The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010]