Rat anti-Mouse PECAM-1 Monoclonal Antibody | anti-PECAM-1 antibody

PECAM-1, Mouse, mAb MEC7.46

Cat. Num. AAA584205

• Gene Names: PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation
• Synonyms: PECAM-1; Monoclonal Antibody; Mouse; mAb MEC7.46; CD31; platelet-endothelial cell adhesion molecule-1; anti-PECAM-1 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 738

• Applicable Applications for anti-PECAM-1 antibody: F, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: Application Notes: FC: Antibody MEC7.46 stains the extracellular domain of mouse PECAM-1; 106 cells were used per sample. No fixation or permeabilization needed. Epitope is sensitive to trypsin! (Ref.1); Application Use: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
• Immunogen: Mouse t.end.1 cells (polyoma middle T (PmT)-transformed EC)
• Positive Control: Mouse t-end.1 cells
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-PECAM-1 antibody

Product Description: The monoclonal antibody MEC7.46 recognizes the mouse form of the platelet-endothelial cell adhesion molecule (PECAM)-1 (CD31). PECAM-1 is a member of the immunoglobulin superfamily. This heavily glycosylated protein is found in the entire vascular endothelium of adult mice and functions in mediating cellular adhesion by heterophilic and homophilic mechanisms. PECAM-1 is detected within the lymphopoietic islands in the spleen of newborn (day 12) and in the bone marrow of adult mice. Capillary endothelial cells of adult mice also express PECAM-1.
The reactivity of the monoclonal antibody MEC7.46 is restricted to the isoform of the molecule that is selectively expressed by endothelial cells. The antibody precipitates a 130 kDa molecule present on the membrane of endothelial cells of all mouse blood vessels both in normal, inflamed and tumor tissues. The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1. Staining of MEC7.46 can be seen on capillaries, veins, arteries and liver sinusoids.

References

1. Vecchi, A et al; Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium. Eur J Cell Biol 1994, 63: 247 2. Xu, Q et al; Circulating progenitor cells regenerate endothelium of vein graft atherosclerosis, which is diminished in ApoE-deficient mice. Circ Res 2003, 93: e76
3. Smetsers, T et al; Localization and characterization of melanoma-associated glycosaminoglycans: Differential expression of chondroitin and heparan sulfate epitopes in melanoma. Canc Res 2003, 63: 2965

NCBI and Uniprot Product Information

• NCBI GI #: 189776
• NCBI GeneID: 5175
• Molecular Weight: 81,378 Da
• NCBI Official Full Name: PECAM-1
• NCBI Official Synonym Full Names: platelet/endothelial cell adhesion molecule 1
• NCBI Official Symbol: PECAM1
• NCBI Official Synonym Symbols: CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• NCBI Protein Information: platelet endothelial cell adhesion molecule; CD31 antigen
• UniProt Protein Name: Platelet endothelial cell adhesion molecule
• UniProt Gene Name: PECAM1
• UniProt Synonym Gene Names: PECAM-1
• UniProt Entry Name: PECA1_HUMAN

NCBI Description

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

Uniprot Description

PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: extracellular space; platelet alpha granule membrane; integral to membrane; plasma membrane; cell junction

Molecular Function: protein binding

Biological Process: platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; diapedesis; cell recognition; cell adhesion; signal transduction; blood coagulation; leukocyte migration; phagocytosis