Mouse anti-Human PDZK1 Monoclonal Antibody | anti-PDZK1 antibody

Mouse Monoclonal [clone 1C3-2B11] (IgG1,k) to Human PDZK1

Cat. Num. AAA246465

• Gene Names: PDZK1; CAP70; CLAMP; PDZD1; NHERF3; NHERF-3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: PDZK1; Monoclonal Antibody; Mouse Monoclonal [clone 1C3-2B11] (IgG1; k) to Human PDZK1; Anti-PDZK1 Antibody (clone 1C3-2B11) IHC-plus; CAP70; CLAMP; NHERF-3; PDZD1; PDZ domain containing 1; NaPi-Cap1; NHERF3; Human PDZK1; anti-PDZK1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1C3-2B11
• Specificity: Human PDZK1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 519

• Applicable Applications for anti-PDZK1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using tissue lysates.
• Target Species: Human
• Immunogen Description: PDZK1 (AAH06496, 1 a.a. ~ 520 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: PDZK1 antibody was raised against pDZK1 (AAH06496, 1 a.a. ~ 520 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PDZK1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(PDZK1 monoclonal antibody. Western blot of PDZK1 expression in human kidney.)

ELISA (EIA)

(Detection limit for recombinant GST tagged PDZK1 is approximately 0.03 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 21361142
• NCBI GeneID: 5174
• NCBI Accession #: NP_002605.2
• NCBI GenBank Nucleotide #: NM_002614.4
• UniProt Accession #: Q5T2W1; O60450; Q5T5P6; Q9BQ41; B4DPB9; E7EU02
• Molecular Weight: 44,665 Da
• NCBI Official Full Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF3 isoform 1
• NCBI Official Synonym Full Names: PDZ domain containing 1
• NCBI Official Symbol: PDZK1
• NCBI Official Synonym Symbols: CAP70; CLAMP; PDZD1; NHERF3; NHERF-3
• NCBI Protein Information: Na(+)/H(+) exchange regulatory cofactor NHE-RF3; Na(+)/H(+) exchange regulatory cofactor NHE-RF3; naPi-Cap1; PDZ-containing kidney protein 1; CFTR-associated protein of 70 kDa; sodium-hydrogen exchanger regulatory factor 3; na/Pi cotransporter C-terminal-associated protein 1
• UniProt Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF3
• Protein Family: PDZK1-interacting protein
• UniProt Gene Name: PDZK1
• UniProt Synonym Gene Names: CAP70; NHERF3; PDZD1; NHERF-3; NaPi-Cap1
• UniProt Entry Name: NHRF3_HUMAN

NCBI Description

This gene encodes a PDZ domain-containing scaffolding protein. PDZ domain-containing molecules bind to and mediate the subcellular localization of target proteins. The encoded protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Pseudogenes of this gene are located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

PDZK1: A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function. Belongs to the NHER family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: brush border membrane; microvillus membrane; apical plasma membrane; cytoplasm; plasma membrane; lipid raft

Molecular Function: protein binding; scavenger receptor binding; transporter activity; protein complex binding; PDZ domain binding

Biological Process: cell proliferation; drug transport; transport; carnitine transport