PDL2 antibody set

PDL2 Detection Set (Risk Free)

Cat. Num. AAA154700

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Antibodies are supplied as protein A purified IgG1
• Synonyms: PDL2; Monoclonal Antibody Set; PDL2 Detection Set (Risk Free); PDL2 antibody set

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: Antibodies are supplied as protein A purified IgG1
• Form/Format: Liquid. PBS containing 0.02% sodium azide.
• Concentration: Antibody 1mg/mL (varies by lot)

• Applicable Applications for PDL2 antibody set: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)-Paraffin, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Immunogen: Mouse monoclonal PD-L2 antibodies were raised against the extracellular domain of human PD-L2.
• Conjugation: Unconjugated
• Preparation and Storage: Antibodies can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Related Product Information for PDL2 antibody set

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PD-L2 can act to stimulate an immunogenic response through an alternative receptor from PD-1. Risk-Free antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-Free means they are guaranteed to work for their approved applications

References

Holling , Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.
Zhang Y, Chung Y, Bishop C, et al. Regulation of T cell activation and tolerance by PDL2. Proc. Natl. Acad. Sci. USA 2006; 103:11695-700.