Mouse PCNA Monoclonal Antibody | anti-PCNA antibody

PCNA (PC10) Antibody

Cat. Num. AAA440084

• Gene Names: PCNA; ATLD2
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Synonyms: PCNA; Monoclonal Antibody; PCNA (PC10) Antibody; anti-PCNA antibody

• Host: Mouse
• Clonality: Monoclonal
• Specificity: Detects PCNA of mouse, rat, human, insect and S. pombe origin
• Form/Format: 100 ug/ml of mouse monoclonal IgG2a in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
• Concentration: 0.100 mg/ml (varies by lot)
• Sequence Length: 261

• Applicable Applications for anti-PCNA antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western blotting starting dilution 1:500.
• Origin: PCNA (PC10) is a mouse monoclonal IgG2a derived from the fusion of spleen cells, from a BALB/c mouse immunized with recombinant PCNA, with Sp2/0-Ag14 myeloma cells.
• Immunogen: Recombinant PCNA
• Preparation and Storage: Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

Related Product Information for anti-PCNA antibody

The evolutionarily conserved protein PCNA, or cyclin, was first characterized as a DNA polymerase accessory protein. It functions as a DNA sliding clamp for DNA polymerase delta and epsilon, and is an key component of for eukaryotic chromosomal DNA replication (1-3). In more recent research PCNA has been linked to an increasing number of cellular functions, these include cell cycle regulation, DNA repair, Okazaki fragment joining, DNA methylation, and chromatin assembly (2,4, &5). In cell cycle regulation p21 interacts with PCNA, which inhibits DNA replication (2,5). It has also been described that high levels of PCNA in the presence of p53 will lead to DNA repair (4). PCNA is also a very useful marker of cellular proliferation as it is seen most prominently expressed during the S phase of the cell cycle (6).

References

1. Kelman Z. 1997. PCNA: structure, functions and interactions. Oncogene 14(6):629-40.2. Tsurimoto T. 1999. PCNA binding proteins. Front Biosci 4:D849-D858.3. Balajee A.S., Geard C.R. 2001. Chromatin-bound PCNA complex formation triggered by DNA damage occurs independent of the ATM gene product in human cells. Nucleic Acid Res 29(6):1341-1351.4. Pauneshku T., Mittal S., Protic M., Oryhon J., Korolev S.V., Joachimiak A., Woloschak G.E. 2001. Proliferating cell nuclear antigen (PCNA): ringmaster of the genome. Int J Radiat Biol 77(10):1007-1021.5. Rossig L., Jadidi A.S., Urbich C., Badorff C., Zeiher A.M., Dimmeler S. 2001. Akt-dependent phosphorylation of p21(Cip1) regulates PCNA binding and proliferation of endothelial cells. Mol Cell Biol 21(16):5644-5657.6. Celis J.E., Madsen P., Nielsen S., Celis A. 1986. Nuclear patterns of cyclin (PCNA) antigen distribution subdivide S-phase in cultured cells?some applications of PCNA antibodies. Leuk Res 10(3):237-249.

NCBI and Uniprot Product Information

• NCBI GI #: 129694
• NCBI GeneID: 5111
• UniProt Accession #: P12004; B2R897; D3DW02
• Molecular Weight: 28,769 Da
• NCBI Official Full Name: Proliferating cell nuclear antigen
• NCBI Official Synonym Full Names: proliferating cell nuclear antigen
• NCBI Official Symbol: PCNA
• NCBI Official Synonym Symbols: ATLD2
• NCBI Protein Information: proliferating cell nuclear antigen; DNA polymerase delta auxiliary protein; cyclin
• UniProt Protein Name: Proliferating cell nuclear antigen
• Protein Family: PCNA-associated factor
• UniProt Gene Name: PCNA
• UniProt Synonym Gene Names: PCNA
• UniProt Entry Name: PCNA_HUMAN

NCBI Description

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Uniprot Description

PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family.

Protein type: DNA replication; Cell cycle regulation

Chromosomal Location of Human Ortholog: 20pter-p12

Cellular Component: nucleoplasm; centrosome; PCNA complex; cytoplasm; DNA replication factor C complex; nuclear replication fork; nucleus

Molecular Function: identical protein binding; protein binding; DNA polymerase processivity factor activity; purine-specific mismatch base pair DNA N-glycosylase activity; MutLalpha complex binding; dinucleotide insertion or deletion binding; receptor tyrosine kinase binding

Biological Process: mismatch repair; positive regulation of deoxyribonuclease activity; heart development; DNA strand elongation during DNA replication; response to lipid; leading strand elongation; DNA repair; telomere maintenance via semi-conservative replication; G1/S-specific transcription in mitotic cell cycle; cell proliferation; bypass DNA synthesis; response to cadmium ion; epithelial cell differentiation; base-excision repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; telomere maintenance; G1/S transition of mitotic cell cycle; regulation of DNA replication

Disease: Ataxia-telangiectasia-like Disorder 2