Western Blot (WB)
(Western blot analysis of PCNA expression in K562 (A), HEK293 (B), A431 (C) whole cell lysates.)
Mouse anti-Human PCNA Monoclonal Antibody | anti-PCNA antibody
Anti-PCNA Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
PCNA; Monoclonal Antibody; Anti-PCNA Antibody; Proliferating cell nuclear antigen; Cyclin; anti-PCNA antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Specificity
Recognizes endogenous levels of PCNA protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Applicable Applications for anti-PCNA antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1/500-1/1000
IHC: 1/100-1/300
IHC: 1/100-1/300
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within human PCNA. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of PCNA expression in K562 (A), HEK293 (B), A431 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of PCNA expression in K562 (A), HEK293 (B), A431 (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PCNA staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PCNA staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-PCNA antibody
Mouse monoclonal antibody to PCNA
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,769 Da
NCBI Official Full Name
proliferating cell nuclear antigen
NCBI Official Synonym Full Names
proliferating cell nuclear antigen
NCBI Official Symbol
PCNA
NCBI Protein Information
proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein
UniProt Protein Name
Proliferating cell nuclear antigen
Protein Family
UniProt Gene Name
PCNA
UniProt Synonym Gene Names
PCNA
UniProt Entry Name
PCNA_HUMAN
Similar Products
Product Notes
The PCNA pcna (Catalog #AAA8309774) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-PCNA Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCNA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1/500-1/1000 IHC: 1/100-1/300. Researchers should empirically determine the suitability of the PCNA pcna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCNA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
