Testing Data
(Western blot analysis of HeLa Nuclear Extract probed with Mouse anti Human PCNA (MBS212237))
Mouse PCNA Monoclonal Antibody | anti-PCNA antibody
MOUSE ANTI PCNA:FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10; Application Note: Membrane permeabilization is required for this application. MyBioSource recommend the use of Leucoperm
1% Bovine Serum Albumin
Preparation
Shelf Life: 18 months from date of despatch.
Testing Data
(Western blot analysis of HeLa Nuclear Extract probed with Mouse anti Human PCNA (MBS212237))
Testing Data
(Western blot analysis of HeLa Nuclear Extract probed with Mouse anti Human PCNA (MBS212237))
Testing Data
(Staining of KM-H2 cells with Mouse anti PCNA:FITC permeabilised with Leucoperm and methanol)
Testing Data
(Staining of KM-H2 cells with Mouse anti PCNA:FITC permeabilised with Leucoperm and methanol)
Testing Data
(Western blot analysis of K562 erythroleukemic cell line whole cell lysate probed with Mouse anti Human PCNA antibody (MBS212209) followed by HRP conjugated Goat anti Mouse IgG secondary antibody, visualized using chemiluminescence)
Testing Data
(Western blot analysis of K562 erythroleukemic cell line whole cell lysate probed with Mouse anti Human PCNA antibody (MBS212209) followed by HRP conjugated Goat anti Mouse IgG secondary antibody, visualized using chemiluminescence)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
Uniprot Description
PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family.
Protein type: Cell cycle regulation; DNA replication
Chromosomal Location of Human Ortholog: 20pter-p12
Cellular Component: nucleoplasm; centrosome; PCNA complex; nuclear replication fork; cytoplasm; DNA replication factor C complex; nucleus
Molecular Function: identical protein binding; protein binding; DNA polymerase processivity factor activity; MutLalpha complex binding; purine-specific mismatch base pair DNA N-glycosylase activity; dinucleotide insertion or deletion binding; receptor tyrosine kinase binding
Biological Process: mismatch repair; positive regulation of deoxyribonuclease activity; heart development; DNA strand elongation during DNA replication; DNA repair; response to lipid; leading strand elongation; G1/S-specific transcription in mitotic cell cycle; telomere maintenance via semi-conservative replication; cell proliferation; bypass DNA synthesis; response to cadmium ion; epithelial cell differentiation; nucleotide-excision repair; base-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; telomere maintenance; G1/S transition of mitotic cell cycle; regulation of DNA replication
Disease: Ataxia-telangiectasia-like Disorder 2
Research Articles on PCNA
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Product Notes
The PCNA pcna (Catalog #AAA210727) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PCNA can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS)*. Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10; Application Note: Membrane permeabilization is required for this application. MyBioSource recommend the use of Leucoperm. Researchers should empirically determine the suitability of the PCNA pcna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCNA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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