Mouse Paxillin Monoclonal Antibody | anti-PXN antibody

Paxillin (PXN) (AP)

Cat. Num. AAA6241538

• Reactivity: Bovine, Chicken, Human, Mouse, Rat
• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Paxillin; Monoclonal Antibody; Paxillin (PXN) (AP); anti-PXN antibody

• Host: Mouse
• Reactivity: Bovine, Chicken, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 0.T.108
• Specificity: Recognizes human Paxillin at 68kD and a band around 50kD, which is most likely a degradation product.; Species Crossreactivity: Human, Mouse, Rat, Chicken and Bovine. Other species not tested.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-PXN antibody: Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ICC: 5-10ug/ml (gave positive immunostaining for paxillin in REF (rat embryo fibroblast) 52 cells fixed with 3.7% paraformaldehyde for 5 minutes).; IP: 4ug per 500ug of cell lysate.; WB: 0.5-2ug/ml detects paxillin in RIPA lysates from 3T3 fibroblasts using 20ug per lane for minigels.; Applications are based on unconjugated antibody.
• Immunogen: GST fusion protein containing full-length human paxillin.
• Conjugate: AP
• Positive Control: 3T3 fibroblasts. Add 2.5ul 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for immunoblot analysis. For use as a positive control for antibodies.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunocytochemistry (ICC)

(ICC: Rat embryo fibroblasts stained with anti-paxillin (10μg/ml))

Product Categories/Family for anti-PXN antibody

Antibodies; Paxillin

References

1. Salgia, R., et al., J. Biol. Chem. 270: 5039-5047, 1995. 2. Salgia, R., et al., J. Biol. Chem. 270: 29145-29150, 1995. 3. Hildebrand, J.D., et al., Mol. Biol. Cell 6: 637-647, 1995.

NCBI and Uniprot Product Information

• NCBI GI #: 170932514
• NCBI GeneID: 5829
• NCBI Accession #: NP_001074324.1
• NCBI GenBank Nucleotide #: NM_002859
• UniProt Accession #: P49023; O14970; O14971; O60360; Q5HYA4; B2RAI3; B7ZMB4
• Molecular Weight: 64,505 Da
• NCBI Official Full Name: paxillin isoform 2
• NCBI Official Synonym Full Names: paxillin
• NCBI Official Symbol: PXN
• NCBI Protein Information: paxillin
• UniProt Protein Name: Paxillin
• Protein Family: Paxillin
• UniProt Gene Name: PXN
• UniProt Entry Name: PAXI_HUMAN

NCBI Description

This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]

Uniprot Description

PXN: a multi-domain cytoskeletal protein involved in actin-membrane attachment that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions. Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the Crk SH2 domain. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.Three alternatively-spliced isoforms have been described. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis; Cell adhesion; Cytoskeletal

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleoplasm; microtubule associated complex; focal adhesion; lamellipodium; cytoplasm; plasma membrane; stress fiber; cell cortex; cytosol

Molecular Function: integrin binding; protein binding; zinc ion binding; beta-catenin binding; BH4 domain binding; protein kinase binding; vinculin binding

Biological Process: epidermal growth factor receptor signaling pathway; lamellipodium biogenesis; focal adhesion formation; integrin-mediated signaling pathway; peptidyl-tyrosine phosphorylation; activation of MAPK activity; signal complex assembly; cytoskeleton organization and biogenesis; signal transduction; regulation of cell shape; branching morphogenesis of a tube; muscle contraction; transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; cell adhesion