Mouse anti-Human Pan Keratin (4,5,6,8,10,13,18) Monoclonal Antibody | anti-KRT5 antibody

Pan Keratin (4,5,6,8,10,13,18)

Cat. Num. AAA395357

• Gene Names: KRT5; K5; CK5; DDD; EBS2; KRT5A
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A/G Chromatography
• Synonyms: Pan Keratin (4; 5; 6; 8; 10; 13; 18); Monoclonal Antibody; anti-KRT5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: C11
• Purity/Purification: Protein A/G Chromatography
• Form/Format: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 590

• Applicable Applications for anti-KRT5 antibody: Western Blot (WB), Immunohistochemistry (IHC) Frozen/Paraffin
• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation from human A431 carcinoma cells and mouse myeloma cells.
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for anti-KRT5 antibody

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.

Product Categories/Family for anti-KRT5 antibody

Monoclonal Antibody; Structural Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 119395754
• NCBI GeneID: 3852
• NCBI Accession #: NP_000415.2
• NCBI GenBank Nucleotide #: NM_000424.3
• UniProt Accession #: P13647; Q6PI71; Q6UBJ0; Q8TA91
• Molecular Weight: 62,378 Da
• NCBI Official Full Name: keratin, type II cytoskeletal 5
• NCBI Official Synonym Full Names: keratin 5
• NCBI Official Symbol: KRT5
• NCBI Official Synonym Symbols: K5; CK5; DDD; EBS2; KRT5A
• NCBI Protein Information: keratin, type II cytoskeletal 5; CK-5; cytokeratin-5; 58 kda cytokeratin; type-II keratin Kb5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types)
• UniProt Protein Name: Keratin, type II cytoskeletal 5
• Protein Family: Keratin
• UniProt Gene Name: KRT5
• UniProt Synonym Gene Names: CK-5; K5
• UniProt Entry Name: K2C5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

Uniprot Description

Subunit structure: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP. Ref.8

Involvement in disease: Epidermolysis bullosa simplex, Dowling-Meara type (DM-EBS) [MIM:131760]: A severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.38Epidermolysis bullosa simplex, with migratory circinate erythema (EBSMCE) [MIM:609352]: A form of intraepidermal epidermolysis bullosa characterized by unusual migratory circinate erythema. Skin lesions appear from birth primarily on the hands, feet, and legs but spare nails, ocular epithelia and mucosae. Lesions heal with brown pigmentation but no scarring. Electron microscopy findings are distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.38Epidermolysis bullosa simplex, Weber-Cockayne type (WC-EBS) [MIM:131800]: A form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.38Epidermolysis bullosa simplex, Koebner type (K-EBS) [MIM:131900]: A form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, although it is less severe.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.12 Ref.18 Ref.25 Ref.28 Ref.31 Ref.32 Ref.38 Ref.39Epidermolysis bullosa simplex, with mottled pigmentation (MP-EBS) [MIM:131960]: A form of intraepidermal epidermolysis bullosa characterized by blistering at acral sites and 'mottled' pigmentation of the trunk and proximal extremities with hyper- and hypopigmentation macules.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.22 Ref.27 Ref.38 Ref.39Dowling-Degos disease (DDD) [MIM:179850]: Autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.38

Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sequence similarities: Belongs to the intermediate filament family.