Immunofluorescence (IF)
(Immunofluorescence staining of Human P4HB in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human P4HB monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to endoplasmic reticulum.)
Rabbit anti-Human P4HB Monoclonal Antibody | anti-P4HB antibody
Recombinant Anti-P4HB Antibody, Rabbit Monoclonal
Gene Names
P4HB; DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; ERBA2L; P4Hbeta
Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A
Synonyms
P4HB; Monoclonal Antibody; Recombinant Anti-P4HB Antibody; Rabbit Monoclonal; P4HB/ERBA2L Antibody; Rabbit MAb; prolyl 4-hydroxylase; beta polypeptide; Anti-DSI Antibody; Anti-ERBA2L Antibody; Anti-GIT Antibody; Anti-P4Hbeta Antibody; Anti-PDI Antibody; Anti-PDIA1 Antibody; Anti-PHDB Antibody; Anti-PO4DB Antibody; Anti-PO4HB Antibody; Anti-PROHB Antibody; anti-P4HB antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
16
Specificity
Human P4HB/ERBA2L
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-P4HB antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
ICC/IF: 1:20-1:100
IP: 1-4uL/mg of lysate
ELISA: 1:5000-1:10,000
ICC/IF: 1:20-1:100
IP: 1-4uL/mg of lysate
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human P4HB protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human P4HB/ERBA2L (rh P4HB).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of Human P4HB in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human P4HB monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to endoplasmic reticulum.)
Immunofluorescence (IF)
(Immunofluorescence staining of Human P4HB in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human P4HB monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to endoplasmic reticulum.)
Immunoprecipitation (IP)
(P4HB was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-P4HB rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-P4HB rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 57 kDaObserved band size: 57 kDa)
Immunoprecipitation (IP)
(P4HB was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-P4HB rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-P4HB rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 57 kDaObserved band size: 57 kDa)
Western Blot (WB)
(Anti-P4HB rabbit monoclonal antibody at 1:100 dilution Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:57 kDa Observed band size:57 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-P4HB rabbit monoclonal antibody at 1:100 dilution Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:57 kDa Observed band size:57 kDa (We are unsure as to the identity of these extra bands.))
Related Product Information for anti-P4HB antibody
Protein disulfide-isomerase, also known as Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55 and P4HB, is a peripheral membrane protein that belongs to the protein disulfide isomerase family. P4HB is highly abundant. In some cell types, it seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. P4HB localizes near CD4-enriched regions on lymphoid cell surfaces. It is identified by mass spectrometry in melanosome fractions from stage I to stage IV. P4HB reduces and may activate fusogenic properties of HIV-1 gp12 surface protein, thereby enabling HIV-1 entry into the cell. P4HB catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. P4HB may therefore cause structural modifications of exofacial proteins. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). P4HB may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
References
Kivirikko KI, et al., 1989, FASEB J., 3 (5): 1609-17. Pihlajaniemi T, et al., 1991, J Hepatol., 13, Suppl 3: S2 Fenouillet E., et al., 2001, J. Infect. Dis. 183:744-752. Gevaert K., et al., 2003, Nat. Biotechnol. 21:566-569. Barbouche R., et al., 2003, J. Biol. Chem. 278:3131-3136.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
508
NCBI Official Full Name
protein disulfide-isomerase
NCBI Official Synonym Full Names
prolyl 4-hydroxylase, beta polypeptide
NCBI Official Symbol
P4HB
NCBI Official Synonym Symbols
DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; ERBA2L; P4Hbeta
NCBI Protein Information
protein disulfide-isomerase; p55; protocollagen hydroxylase; prolyl 4-hydroxylase subunit beta; collagen prolyl 4-hydroxylase beta; thyroid hormone-binding protein p55; glutathione-insulin transhydrogenase; cellular thyroid hormone-binding protein; protei
UniProt Protein Name
Protein disulfide-isomerase
UniProt Gene Name
P4HB
UniProt Synonym Gene Names
ERBA2L; PDI; PDIA1; PO4DB; PDI
UniProt Entry Name
PDIA1_HUMAN
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Product Notes
The P4HB p4hb (Catalog #AAA8104120) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-P4HB Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's P4HB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 ICC/IF: 1:20-1:100 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the P4HB p4hb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "P4HB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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