Mouse p27Kip1 Monoclonal Antibody | anti-CDKN1B antibody

p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Mouse Monoclonal Antibody

Cat. Num. AAA438046

• Gene Names: CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
• Reactivity: Human, Mouse, Rat, Monkey.; Others not known
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Synonyms: p27Kip1; Monoclonal Antibody; p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Mouse Monoclonal Antibody; CDKN1B; CDKN4; Cyclin Dependent Kinase Inhibitor 1B; Cyclin-dependent kinase inhibitor p27 Kip1; KIP1; MEN1B; MEN4; anti-CDKN1B antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Monkey.; Others not known
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: SX53G8
• Specificity: This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 198

• Applicable Applications for anti-CDKN1B antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Western Blot (0.5-1ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Nuclear
• Immunogen: Purified GST-p27 fusion protein of human origin
• Hu-Chromosome Location: 12p13.1
• Positive Control: ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with p27 Monoclonal Antibody (SX53G8))

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with p27 Monoclonal Antibody (SX53G8))

Flow Cytometry (FC/FACS

(Flow Cytometry of human p27 on HeLa Cells.Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled p27 Monoclonal Antibody (SX53G8).)

References

Fredersdorf S, Burns J, Milne AM, Packham G, Fallis L, Gillett CE, et al. High level expression of p27Kip1and cyclin D1 in some human breast cancer cells: Inverse correlation between the expression of p27Kip1 and degree of malignancy in human breast and colorectal cancers. Proc Natl Acad Sci 1997;94:6380-5

NCBI and Uniprot Product Information

• NCBI GI #: 4757962
• NCBI GeneID: 1027
• NCBI Accession #: NP_004055.1
• NCBI GenBank Nucleotide #: NM_004064.4
• UniProt Accession #: P46527; Q16307; Q5U0H2; Q9BUS6
• Molecular Weight: 25-26kDa
• NCBI Official Full Name: cyclin-dependent kinase inhibitor 1B
• NCBI Official Synonym Full Names: cyclin dependent kinase inhibitor 1B
• NCBI Official Symbol: CDKN1B
• NCBI Official Synonym Symbols: KIP1; MEN4; CDKN4; MEN1B; P27KIP1
• NCBI Protein Information: cyclin-dependent kinase inhibitor 1B
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1B
• Protein Family: Cyclin-dependent kinase inhibitor
• UniProt Gene Name: CDKN1B
• UniProt Synonym Gene Names: KIP1
• UniProt Entry Name: CDN1B_HUMAN

NCBI Description

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]

Uniprot Description

p27Kip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. May mediate TGF beta-induced g1 arrest. Its degradation is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Protein type: Oncoprotein; Inhibitor; Protein kinase, regulatory subunit; Cell cycle regulation

Chromosomal Location of Human Ortholog: 12p13.1-p12

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: caspase activator activity; cyclin-dependent protein kinase inhibitor activity; protein binding; protein phosphatase binding; transforming growth factor beta receptor, cytoplasmic mediator activity

Biological Process: autophagic cell death; caspase activation; cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; G1/S transition of mitotic cell cycle; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of kinase activity; negative regulation of mitotic cell cycle; negative regulation of phosphorylation; negative regulation of transcription, DNA-dependent; positive regulation of cell cycle; positive regulation of protein catabolic process; regulation of cyclin-dependent protein kinase activity

Disease: Multiple Endocrine Neoplasia, Type Iv