Mouse anti-Human p21 Monoclonal Antibody | anti-p21 antibody

Mouse anti p21 Monoclonal Antibody

Cat. Num. AAA460245

• Gene Names: CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A-Affinity Chromatography according to Isotyping
• Synonyms: p21; Monoclonal Antibody; Mouse anti p21 Monoclonal Antibody; Cyclin-dependent kinase inhibitor 1A; CDKN1A; P21; P21CIP1; anti-p21 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: This antibody recognizes human p57 protein. It cross reacts to mouse. The other species was not tested.
• Purity/Purification: Purified by Protein A-Affinity Chromatography according to Isotyping
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 164

• Applicable Applications for anti-p21 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: Recombinant human p57/Kip2 protein.
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Related Product Information for anti-p21 antibody

p57/Kip 2 (or CDKN 1C) is a tight-binding strong inhibitor of several G1 cycline/Cdk complexes and a negative regulator of cell proliferation. The gene is a tumor suppressor which is located on chromosome 11p15.5. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndrome (BWS). Several types of childhood tumors including Wilms' tumor, adrenocortical carcinoma and rhabdomyosarcoma display a specific loss of maternal 11p15 alleles, suggesting that genomic imprinting plays an important part. This region also contains two other imprinted genes, insulin like growth factor II (IGF II) and H19, both of which seem to be implicated in adrenal neoplasms.

References

Matsuoka, S., Edwards, M.C., Bai, C., Parker, S., Zhang, P., Baldini, A., Harper, J.W. and Elledge, S.J p57KIP2, a structurally distinct member of the p21CIP1 Cdk inhibitor family, is a candidate tumor suppressor gene. Genes Dev. 9 (6), 650-662 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 453135
• NCBI GeneID: 1026
• Molecular Weight: 18,119 Da
• NCBI Official Full Name: p21
• NCBI Official Synonym Full Names: cyclin dependent kinase inhibitor 1A
• NCBI Official Symbol: CDKN1A
• NCBI Official Synonym Symbols: P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
• NCBI Protein Information: cyclin-dependent kinase inhibitor 1
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1
• Protein Family: P21 prophage-derived protein
• UniProt Gene Name: CDKN1A
• UniProt Synonym Gene Names: CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; MDA-6

NCBI Description

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

p21Cip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. Its expression is tightly controlled by p53, through which this protein mediates the p53-dependent cell cycle arrest at G1 phase.

Protein type: Cell cycle regulation; Inhibitor

Chromosomal Location of Human Ortholog: 6p21.2

Cellular Component: cyclin-dependent protein kinase holoenzyme complex; cytosol; nuclear body; nucleolus; nucleoplasm; nucleus; protein complex

Molecular Function: cyclin-dependent protein kinase activating kinase activity; cyclin-dependent protein kinase activity; cyclin-dependent protein kinase inhibitor activity; protein binding; protein complex binding; protein kinase binding; protein kinase inhibitor activity; ubiquitin protein ligase binding

Biological Process: cell cycle arrest; cellular response to amino acid starvation; cellular response to extracellular stimulus; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of phosphorylation; positive regulation of fibroblast proliferation; positive regulation of protein kinase activity; protein stabilization; Ras protein signal transduction; regulation of cyclin-dependent protein kinase activity; regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus