Mouse P Selectin, Glycoprotein Ligand-1 Monoclonal Antibody | anti-SELPLG antibody

P Selectin, Glycoprotein Ligand-1 (PSGL 1)

Cat. Num. AAA602007

• Gene Names: SELPLG; CLA; CD162; PSGL1; PSGL-1
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Purified; Purified immunoglobulin
• Synonyms: P Selectin; Glycoprotein Ligand-1; Monoclonal Antibody; Glycoprotein Ligand-1 (PSGL 1); Anti -P Selectin; anti-SELPLG antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2Q745
• Specificity: Completely blocks recognition of PSGL-1 by either P-selectin or by L-selectin, but does not affect leukocyte recognition of E-selectin.
• Purity/Purification: Purified; Purified immunoglobulin
• Form/Format: Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.

• Applicable Applications for anti-SELPLG antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in FACS, Western Blot and Immunoprecipitation.
• Immunogen: The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SELPLG antibody

Interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of endothelial cells at sites of inflammation. To date, the primary role of PSGL-1 in mediating the interaction between neutrophils and P-selectin has been well documented.

Product Categories/Family for anti-SELPLG antibody

Antibodies; Abs to Ligands

NCBI and Uniprot Product Information

• NCBI GI #: 4506879
• NCBI GeneID: 6404
• NCBI Accession #: NP_002997.1
• NCBI GenBank Nucleotide #: NM_003006.3
• UniProt Accession #: Q14242; Q12775; Q6GTW7; Q8N7J7; A8K2Y0; B4DQC3; Q6ZS88
• Molecular Weight: 43,201 Da
• NCBI Official Full Name: P-selectin glycoprotein ligand 1
• NCBI Official Synonym Full Names: selectin P ligand
• NCBI Official Symbol: SELPLG
• NCBI Official Synonym Symbols: CLA; CD162; PSGL1; PSGL-1
• NCBI Protein Information: P-selectin glycoprotein ligand 1; cutaneous lymphocyte-associated associated antigen
• UniProt Protein Name: P-selectin glycoprotein ligand 1
• Protein Family: P-selectin glycoprotein ligand
• UniProt Gene Name: SELPLG
• UniProt Entry Name: SELPL_HUMAN

NCBI Description

This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.

Uniprot Description

SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; receptor binding

Biological Process: leukocyte tethering or rolling; blood coagulation; cell adhesion; leukocyte adhesive activation; leukocyte migration