Mouse P-Cadherin (CDH3) Monoclonal Antibody | anti-CDH3 antibody

P-Cadherin (CDH3)

Cat. Num. AAA439160

• Gene Names: CDH3; CDHP; HJMD; PCAD
• Reactivity: Human. Others not known.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Synonyms: P-Cadherin (CDH3); Monoclonal Antibody; Cadherin 3 type 1; Cadherin-3; Cadp; Calcium dependent adhesion protein placental; CDH3; CDHP; HJMD; P-cadherin (Placental); PCAD; Placental cadherin; anti-CDH3 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 6A9
• Specificity: Recognizes a protein of 116kDa, identified as P-Cadherin-1 (CDH3). It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congenital hypotrichosis with juvenile macular dystrophy.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 784

• Applicable Applications for anti-CDH3 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FC/FACS: 0.5-1ug/million cells; IF: 1-2ug/ml; WB: 0.5-1.0ug/ml
• Immunogen: A431 trypsinized membranes
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 16q22.1
• Positive Control: A431 or PC-3 cells. Pancreas, Placenta or Prostate
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Parathyroid stained with Chromogranin A Rabbit Recombinant Monoclonal Ab (CHGA/1731R).)

References

Sanders DS, Perry I, Hardy R, Jankowski J. Aberrant P-cadherin expression is a feature of clonal expansion in the gastrointestinal tract associated with repair and neoplasia. J Pathol. 2000 Apr; 190(5):526-30.

NCBI and Uniprot Product Information

• NCBI GI #: 953953841
• NCBI GeneID: 1001
• NCBI Accession #: NP_001304124.1
• NCBI GenBank Nucleotide #: NM_001317195.1
• UniProt Accession #: P22223; Q05DI6; B2R6F4
• Molecular Weight: 116kDa
• NCBI Official Full Name: cadherin-3 isoform 2
• NCBI Official Synonym Full Names: cadherin 3
• NCBI Official Symbol: CDH3
• NCBI Official Synonym Symbols: CDHP; HJMD; PCAD
• NCBI Protein Information: cadherin-3
• UniProt Protein Name: Cadherin-3
• UniProt Gene Name: CDH3
• UniProt Synonym Gene Names: CDHP; P-cadherin

NCBI Description

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene are associated with hypotrichosis with juvenile macular dystrophy and ectodermal dysplasia, ectrodactyly, and macular dystrophy syndrome (EEMS). [provided by RefSeq, Nov 2015]

Uniprot Description

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.