Mouse P-Cadherin Monoclonal Antibody | anti-CDH3 antibody

P-Cadherin (N-terminal region) Antibody

Cat. Num. AAA474273

• Gene Names: CDH3; CDHP; HJMD; PCAD
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA, Immunocytochemistry
• Synonyms: P-Cadherin; Monoclonal Antibody; P-Cadherin (N-terminal region) Antibody; P-Cadherin (N-terminal region); Cadherin P; anti-CDH3 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M596
• Specificity: Clone M596 was purified using Protein A chromatography. The P-cadherin antibody detects a 120kDa* protein corresponding to endogenous P-Cadherin expressed in human A431 cells.
• Form/Format: Mouse monoclonal, protein A purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-CDH3 antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:1000; ICC: 1:100; WB: 1:1000
• Immunogen: Clone (M596) was generated from a recombinant protein containing amino acids in the N-terminal region of human P-Cadherin. This sequence is highly conserved in mouse and rat P-cadherin.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot image of human A431 cells (lanes 1-4). The blots were probed with mouse monoclonals anti-P-Cadherin at 1:250 (lane 1) and 1:500 (lane 2), 1:1000 (lane 3) and 1:2000 (lane 4).)

Related Product Information for anti-CDH3 antibody

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. P-Cadherin (Cadherin-3) is localized in placenta while E-Cadherin (Cadherin-1) and N-Cadherin (Cadherin-2) are found in epithelial and neural tissues, respectively. P-Cadherin is expressed in normal epithelial cells and some cancer cells, and its sequence contains 5 cadherin domains in the extracellular region.

References

Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261.

NCBI and Uniprot Product Information

• NCBI GI #: 14589891
• NCBI GeneID: 1001
• NCBI Accession #: NP_001784.2
• NCBI GenBank Nucleotide #: NM_001793.4
• UniProt Accession #: P22223; Q05DI6; B2R6F4
• Molecular Weight: 91,418 Da
• NCBI Official Full Name: cadherin-3 preproprotein
• NCBI Official Synonym Full Names: cadherin 3, type 1, P-cadherin (placental)
• NCBI Official Symbol: CDH3
• NCBI Official Synonym Symbols: CDHP; HJMD; PCAD
• NCBI Protein Information: cadherin-3; calcium-dependent adhesion protein, placental
• UniProt Protein Name: Cadherin-3
• UniProt Gene Name: CDH3
• UniProt Synonym Gene Names: CDHP; P-cadherin
• UniProt Entry Name: CADH3_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy. [provided by RefSeq, Jul 2008]

Uniprot Description

CDH3: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Interacts with CDCP1. Expressed in some normal epithelial tissues and in some carcinoma cell lines. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cell-cell adherens junction; cytoplasm; integral to membrane; plasma membrane

Molecular Function: calcium ion binding

Biological Process: response to drug; positive regulation of insulin-like growth factor receptor signaling pathway; intercellular junction assembly and maintenance; wound healing; positive regulation of melanin biosynthetic process; Wnt receptor signaling pathway through beta-catenin; retinal homeostasis; negative regulation of transforming growth factor-beta2 production; hair cycle process; cell-cell adhesion; positive regulation of monophenol monooxygenase activity; negative regulation of catagen; visual perception; keratinization; cell adhesion; homophilic cell adhesion

Disease: Ectodermal Dysplasia, Ectrodactyly, And Macular Dystrophy Syndrome; Hypotrichosis, Congenital, With Juvenile Macular Dystrophy