Mouse anti-Chicken Ovalbumin, Denatured Monoclonal Antibody | anti-SERPINB14 antibody
Ovalbumin, Denatured
Reactivity
Chicken
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A/G affinity chromatography.
Synonyms
Ovalbumin; Denatured; Monoclonal Antibody; Anti -Ovalbumin; anti-SERPINB14 antibody
Host
Mouse
Reactivity
Chicken
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
6F163
Specificity
Recognizes denatured chicken ovalbumin (OA). Reacts with OA in denatured or modified form (surface-adsorbed, heat-denatured or reduced). Strong reaction is seen in ELISA with OA directly coated onto the microtiter well. In Dot Blot reacts with native OA adsorbed onto nitrocellulose. In Western Blot (overnight) after SDS-PAGE, reacts with OA in both reduced and non-reduced forms. Epitope differs from O8075-34 and O8075-35 due to lack of mutual inhibition of antigen binding.
Purity/Purification
Affinity Purified
Purified by Protein A/G affinity chromatography.
Purified by Protein A/G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Applicable Applications for anti-SERPINB14 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:12000. (OA antigen coated on plate at 10ug/ml.)
Western Blot: 1:1000
Dilution: ELISA: 1:12000. (OA antigen coated on plate at 10ug/ml.)
Western Blot: 1:1000
Immunogen
Ovalbumin isolated from chicken egg white, formaldehyde treated, and heat denatured by incubation for 1 hr at 110 degree C.
Culture Medium
RPMI 1640 with 10% fetal calf serum
Hybridoma
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-SERPINB14 antibody
Ovalbumin (OA) is isolated from chicken egg white in which it makes up about 60% of solids. Native ovalbumin is a globular monomer of molecular mass 45kD.
Product Categories/Family for anti-SERPINB14 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,881 Da
NCBI Official Full Name
ovalbumin
NCBI Official Symbol
SERPINB14
NCBI Protein Information
ovalbumin; egg albumin; plakalbumin; allergen Gal d II
UniProt Protein Name
Ovalbumin
Protein Family
UniProt Gene Name
SERPINB14
UniProt Entry Name
OVAL_CHICK
Uniprot Description
ovalbumin: Non-inhibitory serpin. Storage protein of egg white. Belongs to the serpin family. Ov-serpin subfamily.
Cellular Component: extracellular space; extracellular region; phagocytic vesicle; cytosol
Molecular Function: serine-type endopeptidase inhibitor activity; calcium ion binding
Research Articles on SERPINB14
Similar Products
Product Notes
The SERPINB14 serpinb14 (Catalog #AAA602482) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Ovalbumin, Denatured reacts with Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Ovalbumin, Denatured can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:12000. (OA antigen coated on plate at 10ug/ml.) Western Blot: 1:1000. Researchers should empirically determine the suitability of the SERPINB14 serpinb14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ovalbumin, Denatured, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
