Western Blot (WB)
Mouse OST48/DDOST Monoclonal Antibody | anti-OST48/DDOST antibody
OST48/DDOST Mouse mAb
Gene Names
DDOST; OST; WBP1; AGER1; CDG1R; GATD6; OST48; OKSWcl45
Reactivity
Homo sapiens (Human)
Applications
Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Synonyms
OST48/DDOST; Monoclonal Antibody; OST48/DDOST Mouse mAb; advanced glycation end-product receptor 1; advanced glycation endproduct receptor 1; AGER1; DDOST 48 kDa subunit; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic); dolichyl-diphosphooligosaccharide-protein glycosyltransferase; dolichyl-diphosphooligosaccharide-protein glycotransf; dolichyl-diphosphooligosaccharide-protein glycotransferase; OKSWcl45; oligosaccharyl transferase 48 kDa subunit; oligosaccharyltransferase 48 kDa subunit; oligosaccharyltransferase subunit 48; OST; OST48; WBP1; anti-OST48/DDOST antibody
Host
Mouse
Reactivity
Homo sapiens (Human)
Clonality
Monoclonal
Isotype
IgG
Sequence Length
456
Applicable Applications for anti-OST48/DDOST antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200
For Western blot, incubate membrane with diluted antibody in 5% w/v non fat dry milk, 1X TBS, 0.05% Tween -20 at 4°C with gentle shaking overnight
IP: 10 tests
FC/FACS: 1:100-1:200
For Western blot, incubate membrane with diluted antibody in 5% w/v non fat dry milk, 1X TBS, 0.05% Tween -20 at 4°C with gentle shaking overnight
Source Note
This monoclonal antibody is produced by immunizing mice with a polypeptide corresponding to OST48 protein.
Preparation and Storage
Store at -20°C. Stable for one year from the date of shipment.
Western Blot (WB)
Western Blot (WB)
Immunoprecipitation (IP)
Immunoprecipitation (IP)
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Related Product Information for anti-OST48/DDOST antibody
This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts, which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.
Product Categories/Family for anti-OST48/DDOST antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
NCBI Official Synonym Full Names
dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit
NCBI Official Symbol
DDOST
NCBI Official Synonym Symbols
OST; WBP1; AGER1; CDG1R; GATD6; OST48; OKSWcl45
NCBI Protein Information
dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
UniProt Protein Name
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
UniProt Gene Name
DDOST
UniProt Synonym Gene Names
KIAA0115; OST48; DDOST 48 kDa subunit; Oligosaccharyl transferase 48 kDa subunit
NCBI Description
This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq, Jul 2008]
Uniprot Description
Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Required for the assembly of both SST3A- and SS3B-containing OST complexes. Required for efficient N-glycosylation.
Research Articles on OST48/DDOST
Similar Products
Product Notes
The OST48/DDOST ddost (Catalog #AAA435254) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The OST48/DDOST Mouse mAb reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's OST48/DDOST can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:5000 IP: 10 tests FC/FACS: 1:100-1:200 For Western blot, incubate membrane with diluted antibody in 5% w/v non fat dry milk, 1X TBS, 0.05% Tween -20 at 4°C with gentle shaking overnight. Researchers should empirically determine the suitability of the OST48/DDOST ddost for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OST48/DDOST, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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