Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Mouse O-GalNAc Monoclonal Antibody | anti-O-GalNAc antibody
O-GalNAc Antibody, Clone 9B9: ATTO 390
Reactivity
Species Independent
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, ELISA
Purity
PEG Purified
Synonyms
O-GalNAc; Monoclonal Antibody; O-GalNAc Antibody; Clone 9B9: ATTO 390; Mouse Anti-beta-O-Linked N-Acetylgalactosamine Monoclonal IgM; O-GalNAc Antibody: ATTO 390; GalNAC Antibody; O-GalNAC Antibody; beta-O-Linked N-Acetylgalactosamine Antibody; O-Linked N-Acetylgalactosamine Antibody; N-Acetylgalactosamine Antibody; 2-(Acetylamino)-2-deoxy-D-galactose Antibody; 2-Acetamido-2-deoxy-D-galactose Antibody; N-Acetylchondrosamine Antibody; 2-Acetamido-2-deoxy-D-galactopyranose Antibody; N-Acetyl-D-galactosamine Antibody; alpha-N-Acetylgalactosamine Antibody; N-Acetylgalactosamine-modified protein Antibody; anti-O-GalNAc antibody
Host
Mouse
Reactivity
Species Independent
Clonality
Monoclonal
Isotype
IgM
Clone Number
9B9
Specificity
Specific for beta-O-Linked N-Acetylgalactosamine and beta-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with Glucose or Galactose modified proteins.
Purity/Purification
PEG Purified
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-O-GalNAc antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:1000
ICC/IF: 1:50
ELISA: 1:1000
ICC/IF: 1:50
ELISA: 1:1000
Target
O-GalNAC
Immunogen
Synthetic beta-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
ATTO 390
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Preparation and Storage
Store at -20 degree C.
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Western Blot (WB)
(Western Blot analysis of Glycoconjugates showing detection of 67 kDa O-GalNAC protein using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Lane 1: Molecular Weight Ladder (MW). Lane 2: GlcNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA. Load: 2.0 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.)
Western Blot (WB)
(Western Blot analysis of Glycoconjugates showing detection of 67 kDa O-GalNAC protein using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Lane 1: Molecular Weight Ladder (MW). Lane 2: GlcNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA. Load: 2.0 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.)
Product Categories/Family for anti-O-GalNAc antibody
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Product Notes
The O-GalNAc (Catalog #AAA808557) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The O-GalNAc Antibody, Clone 9B9: ATTO 390 reacts with Species Independent and may cross-react with other species as described in the data sheet. AAA Biotech's O-GalNAc can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA). WB: 1:1000 ICC/IF: 1:50 ELISA: 1:1000. Researchers should empirically determine the suitability of the O-GalNAc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "O-GalNAc, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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